Detailed information NTG0446142

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: HPK20_RS13460
• Genome accession: NZ_CP053664
• Coordinates: 2893954..2894958 (-)
• Length: 334 a.a.
• NCBI ID: WP_171933977.1
• Uniprot ID: -
• Organism: Vibrio fluvialis strain A8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2888954..2899958

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HPK20_RS13435 (HPK20_13455)	aroK	2889822..2890340 (-)	519	WP_004728569.1	shikimate kinase AroK	-
HPK20_RS13440 (HPK20_13460)	pilQ	2890528..2892264 (-)	1737	WP_233921186.1	type IV pilus secretin PilQ	Machinery gene
HPK20_RS13445 (HPK20_13465)	pilP	2892289..2892801 (-)	513	WP_020329446.1	pilus assembly protein PilP	Machinery gene
HPK20_RS13450 (HPK20_13470)	pilO	2892791..2893381 (-)	591	WP_171933976.1	type 4a pilus biogenesis protein PilO	Machinery gene
HPK20_RS13455 (HPK20_13475)	pilN	2893374..2893964 (-)	591	WP_100267596.1	PilN domain-containing protein	Machinery gene
HPK20_RS13460 (HPK20_13480)	pilM	2893954..2894958 (-)	1005	WP_171933977.1	type IV pilus assembly protein PilM	Machinery gene
HPK20_RS13465 (HPK20_13485)	-	2895101..2897581 (+)	2481	WP_032081685.1	penicillin-binding protein 1A	-
HPK20_RS13470 (HPK20_13490)	oxyR	2897642..2898538 (-)	897	WP_020329438.1	DNA-binding transcriptional regulator OxyR	-
HPK20_RS13475 (HPK20_13495)	-	2898682..2899416 (+)	735	WP_024374097.1	glutathione peroxidase	-
HPK20_RS13480 (HPK20_13500)	-	2899545..2899808 (+)	264	WP_158125472.1	DUF3624 domain-containing protein	-

Sequence

Protein

• Length: 334 a.a.
• Molecular weight: 36863.20 Da
• Isoelectric Point: 6.6961

>NTDB_id=446142 HPK20_RS13460 WP_171933977.1 2893954..2894958(-) (pilM) [Vibrio fluvialis strain A8]
MGKSLVTGVDIGHHSIKAVVLKPKGESYSLVGYQEIVVSEDIFADNHTLNYQKIVKKLKELKKGLPLFSRKVALAVPDNA
VISKVLQIDSELDASEQEFALYQAFSHQSPLPIEELSLDFVVTGEKTLGRSSSLTYQVYATRREVIDNRLSAMSKAGFEP
LLFDMQIHGLLNIWQWVSRAQQRTDWMLVDVGFSQTSLCIDFADKTPFYKDIPLGTRQLVAATSGGNADLTSPQAHFIQE
LVERLARQIQLFLSLQGHQPLAGIWLSGGGAATEGLAQAISERLALPCELFNPLAEFTCRASLRRQPQADLQRFSTAAGL
ALRGLNWLENGHAA

Nucleotide

• Length: 1005 bp

>NTDB_id=446142 HPK20_RS13460 WP_171933977.1 2893954..2894958(-) (pilM) [Vibrio fluvialis strain A8]
ATGGGTAAATCATTAGTTACAGGTGTCGATATTGGCCACCACAGTATTAAAGCGGTGGTTTTAAAACCGAAAGGCGAATC
GTATTCGCTGGTCGGTTACCAAGAGATAGTCGTATCCGAGGATATTTTCGCTGATAACCACACGCTGAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGATTACCGCTATTTAGTCGTAAAGTGGCGCTGGCTGTACCGGACAACGCA
GTAATAAGTAAGGTATTACAGATAGATAGCGAACTGGATGCCAGCGAACAAGAGTTCGCGCTCTATCAGGCTTTTTCCCA
TCAATCGCCGCTACCCATCGAAGAGTTGAGCCTGGATTTCGTTGTCACCGGAGAAAAAACACTGGGGCGCAGCAGTTCGT
TGACGTATCAGGTCTATGCAACCCGACGTGAAGTGATCGACAACCGTTTGTCTGCGATGAGCAAAGCGGGTTTTGAGCCG
CTGCTGTTTGATATGCAGATTCACGGCCTGCTCAATATCTGGCAATGGGTCAGCCGTGCTCAGCAACGCACCGATTGGAT
GCTGGTCGATGTCGGGTTCAGCCAAACCTCATTGTGCATCGACTTTGCCGATAAAACGCCGTTTTACAAAGACATTCCGT
TGGGAACCCGCCAACTGGTTGCGGCGACCTCCGGTGGTAACGCTGACCTTACCAGCCCGCAAGCACACTTCATTCAGGAG
TTGGTTGAGCGTCTGGCGCGCCAGATCCAGCTGTTTCTTTCTCTGCAAGGCCATCAACCGCTGGCCGGTATCTGGCTATC
CGGTGGCGGCGCGGCAACGGAAGGTTTGGCGCAGGCGATTAGCGAACGTCTGGCTCTGCCTTGCGAGCTGTTTAACCCTC
TGGCGGAGTTTACTTGCCGCGCATCGCTACGCCGCCAGCCTCAGGCTGATTTGCAGCGTTTCAGTACTGCGGCCGGCTTG
GCGCTGCGCGGCCTGAATTGGCTGGAGAACGGCCATGCTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (237-324)

• Type IV pilus assembly protein PilM; (PilM_2) (8-219)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	65.089	100	0.659
pilM	Vibrio campbellii strain DS40M4	48.116	100	0.497