Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   I3J23_RS17660 Genome accession   NZ_CP065159
Coordinates   3467971..3469914 (+) Length   647 a.a.
NCBI ID   WP_025851308.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain GXU-1     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 3462971..3474914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3J23_RS17635 (I3J23_17635) def 3463346..3463828 (+) 483 WP_003154343.1 peptide deformylase -
  I3J23_RS17640 (I3J23_17640) fmt 3463833..3464786 (+) 954 WP_014304991.1 methionyl-tRNA formyltransferase -
  I3J23_RS17645 (I3J23_17645) rsmB 3464773..3466116 (+) 1344 WP_003154341.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  I3J23_RS17650 (I3J23_17650) rlmN 3466119..3467210 (+) 1092 WP_003154339.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  I3J23_RS17655 (I3J23_17655) - 3467216..3467977 (+) 762 WP_014304992.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  I3J23_RS17660 (I3J23_17660) stkP 3467971..3469914 (+) 1944 WP_025851308.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  I3J23_RS17665 (I3J23_17665) rsgA 3469929..3470819 (+) 891 WP_003154333.1 ribosome small subunit-dependent GTPase A -
  I3J23_RS17670 (I3J23_17670) rpe 3470821..3471474 (+) 654 WP_003154331.1 ribulose-phosphate 3-epimerase -
  I3J23_RS17675 (I3J23_17675) - 3471540..3472184 (+) 645 WP_015388245.1 thiamine diphosphokinase -
  I3J23_RS17680 (I3J23_17680) spoVM 3472271..3472351 (+) 81 WP_003154329.1 stage V sporulation protein SpoVM -
  I3J23_RS17685 (I3J23_17685) rpmB 3472429..3472617 (-) 189 WP_003154328.1 50S ribosomal protein L28 -
  I3J23_RS17690 (I3J23_17690) - 3472876..3473238 (+) 363 WP_003154327.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 647 a.a.        Molecular weight: 71727.26 Da        Isoelectric Point: 4.9835

>NTDB_id=445860 I3J23_RS17660 WP_025851308.1 3467971..3469914(+) (stkP) [Bacillus amyloliquefaciens strain GXU-1]
MLTGKRISGRYHILRPIGGGGMANVFLAEDIILEREVAIKILRFDFVNDIDFIRRFRREAQSASSLDHPNIVSIYDIGEE
GDIYYIVMEYVEGMTLKEYINAHGPLHPKEALSVMEQIVSAIAHAHQNHIVHRDIKPHNILIDHLGHIKVTDFGIATALT
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGKIPFDGESAVSIALKHLQTETPSARKWNPSIPQS
VENIILKATAKDPFHRYESAEDMEADIRTAFDAGRLNEQKFSVQDDEEMTKAIPVITDGPKAASQAETPGETDDKEQQPA
KKKKRKWPWVLLAVCFIFIMAAVLAVTVFPSLFMPKDVSVPDVRGMKYEKAEALLEKNGLRADPDITDIEDEKIEEELMV
KTDPKAGSTVKEGSSVKLYKSIGKPKTQLIDVKGRQIGDAKKALKEKGFKHVNVKEENDDSEAGTVIDQDPSAGTDMVAS
DDEVNLTVSLGPADVTLRDLKTYSKEAASGYLEDNGLQLVEKQAHSDDVPEGQVMKQEPSAGTAVKPGSNVEVTFSLGPE
EKPAKTVKEKISIPYEPEHEGDELEVQIAIDDKDHSISDTYDSFKIKEPTEKTIELKIDQGQKGYYQVMVDHKVVSYKTI
EYPKDND

Nucleotide


Download         Length: 1944 bp        

>NTDB_id=445860 I3J23_RS17660 WP_025851308.1 3467971..3469914(+) (stkP) [Bacillus amyloliquefaciens strain GXU-1]
GTGCTAACCGGCAAGCGGATCAGCGGGCGTTATCATATCCTCCGGCCCATTGGCGGCGGCGGAATGGCAAACGTTTTTTT
AGCTGAGGACATCATTCTCGAACGTGAAGTCGCAATTAAAATTCTGCGGTTTGACTTTGTGAATGACATTGATTTTATCA
GGCGTTTCAGAAGAGAAGCACAGTCGGCATCAAGCCTCGATCATCCGAATATCGTCAGTATTTACGATATCGGGGAGGAA
GGCGATATTTATTACATTGTCATGGAATATGTGGAAGGCATGACATTAAAGGAATACATAAACGCACACGGACCGCTTCA
TCCGAAAGAAGCGCTGTCCGTTATGGAGCAGATCGTCTCGGCCATCGCCCATGCCCATCAAAACCATATCGTGCACCGGG
ATATTAAACCGCACAATATATTGATAGATCATTTAGGGCATATCAAGGTGACGGATTTCGGCATCGCCACAGCGCTTACG
TCTACGACAATCACACATACGAATTCTGTGCTGGGCTCGGTTCATTATTTGTCTCCGGAGCAGGCGAGAGGCGGTCTGGC
AACGAAAAAATCAGATATTTACGCGCTCGGCATCGTGCTGTTTGAGCTTTTAACAGGCAAAATCCCTTTTGACGGAGAAT
CAGCCGTCAGCATAGCGCTAAAGCACCTGCAAACAGAAACGCCTTCCGCCAGAAAGTGGAACCCTTCCATTCCGCAGAGC
GTTGAAAACATTATTTTAAAGGCAACCGCCAAAGACCCGTTTCACCGCTATGAAAGCGCCGAGGACATGGAGGCGGACAT
CAGAACGGCTTTTGACGCCGGCAGGCTGAATGAACAAAAATTTTCCGTGCAGGATGATGAGGAAATGACAAAAGCCATTC
CCGTTATTACTGACGGCCCAAAAGCCGCGTCTCAGGCGGAAACGCCGGGGGAGACGGATGATAAGGAACAGCAGCCAGCC
AAAAAGAAAAAACGGAAATGGCCGTGGGTTTTGCTGGCCGTCTGTTTCATTTTCATTATGGCGGCTGTTCTTGCGGTCAC
CGTGTTCCCGTCTTTATTTATGCCTAAAGATGTAAGCGTCCCCGACGTACGGGGCATGAAGTACGAAAAAGCGGAAGCCC
TGCTTGAAAAGAACGGGCTCCGGGCTGATCCTGATATAACGGATATTGAAGATGAAAAAATAGAAGAGGAATTAATGGTC
AAAACGGACCCAAAAGCGGGGTCTACCGTTAAAGAAGGCTCTTCTGTAAAACTGTACAAAAGCATCGGAAAACCGAAAAC
CCAGCTGATCGACGTGAAAGGACGGCAGATCGGCGACGCAAAAAAAGCGCTGAAGGAAAAAGGTTTCAAACATGTCAATG
TCAAAGAAGAAAACGATGACAGCGAGGCCGGCACCGTAATTGATCAGGACCCGTCAGCCGGCACCGATATGGTCGCAAGC
GACGATGAAGTCAATCTAACGGTCAGCCTCGGCCCGGCGGACGTTACGCTGAGGGACTTAAAGACTTACAGCAAAGAAGC
GGCTTCAGGGTATCTTGAAGATAACGGCCTGCAGCTTGTTGAAAAACAAGCCCATTCAGATGACGTGCCGGAAGGACAAG
TCATGAAACAAGAGCCGTCAGCCGGCACTGCGGTTAAGCCGGGAAGTAATGTTGAAGTGACGTTCTCGCTCGGACCTGAA
GAAAAACCGGCGAAAACGGTAAAAGAAAAAATCAGCATTCCTTACGAACCCGAACATGAAGGGGACGAGCTTGAAGTGCA
AATCGCCATCGATGATAAGGACCACAGCATCTCTGATACGTATGACTCATTTAAAATAAAAGAGCCGACCGAAAAAACAA
TTGAACTGAAGATTGATCAGGGACAGAAGGGGTATTATCAAGTGATGGTCGATCATAAAGTCGTCAGCTACAAAACGATT
GAATACCCGAAAGATAATGACTGA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(431-489)

PASTA domain (PASTA)

(495-556)

PASTA domain (PASTA)

(358-419)

Protein kinase domain (Pkinase)

(11-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

38.961

95.209

0.371
  stkP Streptococcus pneumoniae D39

38.799

95.209

0.369
  stkP Streptococcus pneumoniae R6

38.799

95.209

0.369
  stkP Streptococcus pneumoniae Rx1

38.636

95.209

0.368