Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HNI00_RS15800 Genome accession   NZ_CP053540
Coordinates   3738599..3740602 (+) Length   667 a.a.
NCBI ID   WP_172356238.1    Uniprot ID   -
Organism   Thermoleptolyngbya oregonensis NK1-22     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3733599..3745602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNI00_RS15790 (HNI00_15800) dnaK 3734754..3736751 (-) 1998 WP_316787531.1 molecular chaperone DnaK -
  HNI00_RS15795 (HNI00_15805) grpE 3737133..3737828 (-) 696 WP_172356236.1 nucleotide exchange factor GrpE -
  HNI00_RS15800 (HNI00_15810) pilB/pilB1 3738599..3740602 (+) 2004 WP_172356238.1 GspE/PulE family protein Machinery gene
  HNI00_RS15805 (HNI00_15815) pilT 3740707..3741837 (+) 1131 WP_297077565.1 type IV pilus twitching motility protein PilT Machinery gene
  HNI00_RS15810 (HNI00_15820) - 3742166..3743401 (+) 1236 WP_172356242.1 type II secretion system F family protein -
  HNI00_RS15815 (HNI00_15825) - 3743550..3744155 (+) 606 WP_172356244.1 hypothetical protein -
  HNI00_RS15820 (HNI00_15830) - 3744191..3744778 (-) 588 WP_316787532.1 hypothetical protein -
  HNI00_RS15825 (HNI00_15835) - 3744799..3745236 (-) 438 WP_316787533.1 DUF4079 domain-containing protein -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 74504.24 Da        Isoelectric Point: 4.7720

>NTDB_id=445025 HNI00_RS15800 WP_172356238.1 3738599..3740602(+) (pilB/pilB1) [Thermoleptolyngbya oregonensis NK1-22]
MANSSSQRRALVVQHNFSPFSNKLIQAGYVNNDQMQQALVESRKTGRPLTDVLEMITGQQLPPDLLRQYKKQQLFELKIL
YGVESLDPEINEISTAQVGQLIDSLIPVDLCRRYRLVPLSRTESEPPAVLIAMVDPDDLAAQDDLNRILRPQGLGLMRMV
ITVEDYQRIISQYLDEQVERQKQLEFQSKVDVKADLEGLDYLDLEEPPDDIEANLDAALQDADAAPVIALVNKILVKALQ
EGVSDIHIEPQEEHLRIRFRKDGVLREGLEPLPKKIVPAVTARFKIIADLDIAERRMPQDGRIRRIFDGRKVDFRVNTLP
SRYGEKVVLRILDNSATQLGLDKLITDPETLQIVQEMVSRPFGLILVTGPTGSGKTTTLYSALAERNSPGVNISTAEDPI
EYSLPGITQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAIARLEEMGV
ESFMVSSSLIGVLAQRLMRRVCPDCRIPYSPSPEELARFGLSASREAEVTFYKANELSPDEIQSARAQNTLCPTCKGSGY
KGRCGVYEVMRVTEQIQTLITEGAPTERIKEVAVDEGMKTLLAYSLNLVREGSTTLEEVERVTFTDTGLEAELKAKRKSS
LTCRNCAAELRPEWLDCPYCTTPRFQD

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=445025 HNI00_RS15800 WP_172356238.1 3738599..3740602(+) (pilB/pilB1) [Thermoleptolyngbya oregonensis NK1-22]
ATGGCTAACTCTTCATCCCAACGTCGTGCCCTCGTCGTCCAGCATAATTTTTCGCCCTTCAGCAACAAGCTCATCCAGGC
GGGCTACGTCAACAACGACCAGATGCAGCAAGCCCTGGTGGAAAGCCGCAAAACAGGGCGACCGCTGACGGATGTGTTGG
AGATGATTACAGGTCAGCAGTTGCCGCCTGACCTGCTGCGCCAATATAAGAAGCAACAGCTTTTTGAACTTAAAATTCTC
TACGGAGTTGAATCCCTCGATCCAGAGATCAACGAAATTTCGACTGCCCAGGTCGGGCAACTCATCGACAGTCTCATTCC
CGTGGATCTGTGCCGTCGCTATCGCCTTGTGCCTCTGTCGCGCACCGAAAGCGAACCGCCCGCAGTGCTGATTGCAATGG
TCGATCCCGATGACCTGGCTGCACAAGACGACCTCAACCGCATTCTGCGTCCGCAGGGTCTGGGGCTAATGCGGATGGTG
ATCACGGTTGAAGACTATCAGCGGATCATCTCTCAATATCTAGATGAGCAGGTCGAACGCCAGAAGCAGCTTGAGTTTCA
GTCCAAGGTGGATGTGAAGGCAGATCTTGAAGGGCTGGATTACCTCGATCTGGAGGAACCACCCGACGATATCGAAGCCA
ACTTGGATGCTGCCCTTCAGGATGCCGACGCGGCACCGGTGATTGCCTTGGTCAACAAAATTCTGGTGAAGGCGCTCCAG
GAAGGGGTTTCCGATATCCACATCGAGCCGCAGGAAGAACATCTCCGCATCCGCTTTCGCAAAGACGGCGTGCTGCGGGA
GGGTCTGGAGCCGCTGCCCAAGAAAATCGTTCCGGCGGTCACCGCTCGCTTCAAAATCATTGCTGATCTGGACATTGCCG
AACGCCGGATGCCCCAGGACGGACGCATTCGCCGGATTTTTGACGGGCGCAAAGTGGACTTCCGGGTGAACACGCTGCCC
AGCCGCTATGGCGAAAAGGTGGTGCTGCGGATTCTTGACAACTCCGCGACTCAGCTTGGGCTGGACAAACTGATCACCGA
CCCAGAAACGCTGCAAATTGTGCAGGAAATGGTGTCGCGTCCCTTTGGTCTGATCCTGGTGACGGGGCCGACTGGATCGG
GTAAAACGACAACGCTCTACTCTGCGCTGGCGGAGCGCAATAGCCCCGGCGTGAACATCAGCACCGCCGAAGACCCGATT
GAATACTCCCTGCCGGGGATTACCCAGGTGCAGGTGATCCGTGAAAAAGGGATGGACTTTGCCTCGATTCTGCGAGCCTT
CCTGCGGCAAGACCCGGACGTGATTCTGGTGGGTGAGACTCGTGACAAGGAAACGGCGAAGACAGCAATCGAAGCGGCAC
TGACGGGACACCTGGTGCTGACGACGCTGCACACGAATGACGCTGCGAGTGCGATCGCCCGTCTCGAAGAAATGGGCGTA
GAGTCCTTCATGGTGTCTAGTTCGCTGATTGGCGTGCTGGCTCAGCGCCTCATGCGCCGTGTCTGCCCCGACTGCCGTAT
TCCCTACAGCCCTAGCCCCGAAGAACTGGCCCGCTTTGGCCTATCGGCTTCTCGCGAAGCAGAAGTGACCTTCTACAAGG
CCAACGAACTGTCACCTGACGAAATTCAGTCCGCTCGCGCCCAAAACACCCTCTGCCCCACCTGCAAGGGCAGTGGCTAC
AAAGGACGCTGCGGGGTATACGAAGTCATGCGTGTAACGGAGCAAATCCAGACTCTCATCACTGAAGGCGCACCCACCGA
GCGAATCAAGGAAGTGGCAGTAGACGAGGGCATGAAGACCTTGCTGGCCTATAGCCTGAACCTGGTACGCGAGGGCAGTA
CAACGCTTGAAGAGGTAGAGCGCGTCACCTTTACCGATACGGGATTGGAGGCGGAACTCAAAGCCAAGCGCAAGAGTTCG
CTGACTTGTCGCAACTGTGCTGCTGAGCTACGACCTGAATGGCTAGATTGCCCCTATTGCACCACCCCCCGCTTCCAGGA
TTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(57-178)

Type II/IV secretion system protein (T2SSE)

(233-500)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

68.477

99.4

0.681
  pilF Thermus thermophilus HB27

39.32

92.654

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.969

93.103

0.363