Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HNO52_RS19485 Genome accession   NZ_CP053382
Coordinates   4187345..4188379 (-) Length   344 a.a.
NCBI ID   WP_197566822.1    Uniprot ID   A0A7M1QHA4
Organism   Halomonas sp. MCCC 1A13316     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4182345..4193379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNO52_RS19470 (HNO52_19460) tyrS 4183712..4184911 (-) 1200 WP_197566819.1 tyrosine--tRNA ligase -
  HNO52_RS19475 (HNO52_19465) - 4185026..4186156 (+) 1131 WP_197566820.1 anhydro-N-acetylmuramic acid kinase -
  HNO52_RS19480 (HNO52_19470) pilU 4186172..4187314 (-) 1143 WP_197566821.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HNO52_RS19485 (HNO52_19475) pilT 4187345..4188379 (-) 1035 WP_197566822.1 type IV pilus twitching motility protein PilT Machinery gene
  HNO52_RS19490 (HNO52_19480) - 4188458..4189168 (+) 711 WP_197569309.1 YggS family pyridoxal phosphate-dependent enzyme -
  HNO52_RS19495 (HNO52_19485) proC 4189228..4190052 (+) 825 WP_197566823.1 pyrroline-5-carboxylate reductase -
  HNO52_RS19500 (HNO52_19490) - 4190112..4190702 (+) 591 WP_197566824.1 YggT family protein -
  HNO52_RS19505 (HNO52_19495) - 4190754..4191911 (+) 1158 WP_197569310.1 homoserine O-acetyltransferase -
  HNO52_RS19510 (HNO52_19500) metW 4191911..4192513 (+) 603 WP_197569311.1 methionine biosynthesis protein MetW -
  HNO52_RS19515 (HNO52_19505) - 4192551..4193012 (+) 462 WP_197566825.1 DUF4426 domain-containing protein -
  HNO52_RS19520 (HNO52_19510) - 4193025..4193345 (+) 321 WP_197566826.1 Rieske 2Fe-2S domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38102.54 Da        Isoelectric Point: 6.4225

>NTDB_id=444643 HNO52_RS19485 WP_197566822.1 4187345..4188379(-) (pilT) [Halomonas sp. MCCC 1A13316]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVSAMEDKEVRKLIYDIMGDRQRRDYEEFFETDFSFEVPGVAR
FRVNAFNQARGAGAVFRTIPSEVLTMEDLELGDVFRRLSLLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRYDHILTIED
PVEFVHRSKRCLINQREVHRDTHGFAQALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKAMVRSMLSESLQAVISQTLLKRRSGGRVAAHEILIANAAVRNLIREDKVAQIYSAIQTGGNIGMQTLDAAL
AKLVAENVVNRDEAQARAKSALAS

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=444643 HNO52_RS19485 WP_197566822.1 4187345..4188379(-) (pilT) [Halomonas sp. MCCC 1A13316]
ATGGATATTACCGAACTGCTGGCGTTCTCGGCAAAGCAGAACGCCTCCGACCTGCATCTGTCGGCCGGTCTGCCGCCGAT
GATTCGTGTCGATGGCGACATTCGCCGCCTCAACGTGTCGGCCATGGAGGACAAGGAAGTCCGCAAGCTGATCTACGACA
TCATGGGCGACCGGCAGCGTCGTGACTACGAGGAGTTCTTCGAAACCGACTTCTCCTTCGAAGTCCCCGGAGTCGCGCGT
TTCCGGGTCAATGCCTTCAACCAGGCGCGCGGGGCCGGTGCGGTATTCCGCACCATTCCCAGTGAGGTGCTGACCATGGA
GGACCTGGAGCTGGGTGATGTCTTCCGCCGCCTGTCACTGCTGCCACGTGGCCTGGTGTTGGTCACCGGCCCTACCGGCT
CGGGCAAGAGCACGACCTTGGCCGCCATGATCGACTATATCAACGATCACCGCTATGACCATATCCTCACCATCGAGGAC
CCGGTGGAATTCGTCCACCGCAGCAAGCGCTGTCTGATCAACCAGCGTGAGGTGCACCGCGACACCCACGGCTTCGCCCA
GGCGTTGCGCAGCGCCTTGCGCGAGGATCCCGACGTCATCCTGGTCGGTGAGTTGCGCGACCTCGAGACCATTCGCTTGG
CGCTGACTGCCGCCGAAACCGGCCACCTGGTATTCGGTACCCTGCATACCACCTCGGCGGCCAAGACCATCGACCGTATC
ATCGATGTCTTCCCCGGCGAGGAGAAGGCCATGGTGCGCTCGATGCTGTCTGAGTCGCTGCAGGCGGTGATCTCGCAGAC
GCTGCTCAAGCGCCGCAGTGGCGGCCGGGTCGCAGCGCACGAGATCCTCATTGCCAACGCCGCGGTGCGTAACCTGATCC
GCGAGGACAAGGTAGCGCAGATCTATTCGGCCATTCAGACCGGCGGCAATATCGGTATGCAGACTCTCGACGCCGCCCTG
GCCAAGCTGGTGGCCGAAAACGTGGTCAATCGCGACGAGGCGCAGGCCCGCGCCAAGAGCGCGCTGGCAAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7M1QHA4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.056

98.547

0.779
  pilT Pseudomonas aeruginosa PAK

79.056

98.547

0.779
  pilT Acinetobacter baumannii D1279779

78.171

98.547

0.77
  pilT Acinetobacter baumannii strain A118

78.171

98.547

0.77
  pilT Acinetobacter nosocomialis M2

77.876

98.547

0.767
  pilT Acinetobacter baylyi ADP1

77.059

98.837

0.762
  pilT Legionella pneumophila strain Lp02

71.893

98.256

0.706
  pilT Legionella pneumophila strain ERS1305867

71.893

98.256

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.082

99.419

0.657
  pilT Neisseria gonorrhoeae MS11

65.789

99.419

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.982

98.547

0.532
  pilU Pseudomonas stutzeri DSM 10701

41.228

99.419

0.41
  pilU Vibrio cholerae strain A1552

40.643

99.419

0.404
  pilU Acinetobacter baylyi ADP1

39.474

99.419

0.392