Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   IUJ40_RS06345 Genome accession   NZ_CP064389
Coordinates   1225153..1226223 (+) Length   356 a.a.
NCBI ID   WP_000775708.1    Uniprot ID   A0A0H2XFN2
Organism   Staphylococcus aureus strain PartE-Saureus-RM8376     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1220153..1231223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUJ40_RS06315 (IUJ40_06325) - 1220576..1222039 (+) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  IUJ40_RS06320 (IUJ40_06330) - 1222020..1222223 (+) 204 WP_000087561.1 YqgQ family protein -
  IUJ40_RS06325 (IUJ40_06335) - 1222220..1223206 (+) 987 WP_000161314.1 ROK family glucokinase -
  IUJ40_RS06330 (IUJ40_06340) - 1223206..1223535 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IUJ40_RS06335 (IUJ40_06345) - 1223532..1224155 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  IUJ40_RS06340 (IUJ40_06350) comGA 1224207..1225181 (+) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  IUJ40_RS06345 (IUJ40_06355) comGB 1225153..1226223 (+) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  IUJ40_RS06350 (IUJ40_06360) comGC 1226237..1226548 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  IUJ40_RS06355 (IUJ40_06365) comGD 1226526..1226972 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  IUJ40_RS06360 (IUJ40_06370) comGE 1226959..1227258 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  IUJ40_RS06365 (IUJ40_06375) comGF 1227176..1227673 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  IUJ40_RS06370 (IUJ40_06380) - 1227770..1227916 (+) 147 WP_001789879.1 hypothetical protein -
  IUJ40_RS06375 (IUJ40_06385) - 1227906..1228430 (+) 525 WP_001015117.1 shikimate kinase -
  IUJ40_RS06380 (IUJ40_06390) gcvT 1228589..1229680 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  IUJ40_RS06385 (IUJ40_06395) gcvPA 1229700..1231046 (+) 1347 WP_000019693.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41364.77 Da        Isoelectric Point: 10.0092

>NTDB_id=443565 IUJ40_RS06345 WP_000775708.1 1225153..1226223(+) (comGB) [Staphylococcus aureus strain PartE-Saureus-RM8376]
MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=443565 IUJ40_RS06345 WP_000775708.1 1225153..1226223(+) (comGB) [Staphylococcus aureus strain PartE-Saureus-RM8376]
GTGAAACTACAATGCATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGACTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2XFN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.719

100

0.997
  comGB Staphylococcus aureus N315

99.719

100

0.997