Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   HKM25_RS11215 Genome accession   NZ_CP053210
Coordinates   2188795..2190093 (-) Length   432 a.a.
NCBI ID   WP_000867613.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 6A-10     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2183795..2195093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKM25_RS11195 (HKM25_2302) rplI 2184950..2185402 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  HKM25_RS11200 (HKM25_2303) - 2185399..2187372 (-) 1974 WP_170835816.1 DHH family phosphoesterase -
  HKM25_RS11205 (HKM25_2304) raiA 2187508..2188056 (-) 549 WP_000599107.1 ribosome-associated translation inhibitor RaiA -
  HKM25_RS11210 (HKM25_2305) comFC/cflB 2188136..2188798 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  HKM25_RS11215 (HKM25_2306) comFA/cflA 2188795..2190093 (-) 1299 WP_000867613.1 DEAD/DEAH box helicase Machinery gene
  HKM25_RS11220 (HKM25_2307) - 2190149..2190784 (+) 636 WP_000395747.1 YigZ family protein -
  HKM25_RS11225 (HKM25_2308) cysK 2191033..2191959 (+) 927 WP_000029870.1 cysteine synthase A -
  HKM25_RS11230 (HKM25_2309) tsf 2192080..2193120 (-) 1041 WP_000808062.1 translation elongation factor Ts -
  HKM25_RS11235 (HKM25_2310) rpsB 2193199..2193978 (-) 780 WP_000268466.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49608.54 Da        Isoelectric Point: 8.4382

>NTDB_id=443487 HKM25_RS11215 WP_000867613.1 2188795..2190093(-) (comFA/cflA) [Streptococcus pneumoniae strain 6A-10]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGEMTILISTTILERGVTFPCVDVFVVEANHRLFTKSNLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=443487 HKM25_RS11215 WP_000867613.1 2188795..2190093(-) (comFA/cflA) [Streptococcus pneumoniae strain 6A-10]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGTTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAAATGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAATTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

98.843

100

0.988
  comFA/cflA Streptococcus pneumoniae D39

98.843

100

0.988
  comFA/cflA Streptococcus pneumoniae R6

98.843

100

0.988
  comFA/cflA Streptococcus pneumoniae TIGR4

98.611

100

0.986
  comFA/cflA Streptococcus mitis NCTC 12261

93.056

100

0.931
  comFA/cflA Streptococcus mitis SK321

91.898

100

0.919
  comFA Lactococcus lactis subsp. cremoris KW2

51.961

94.444

0.491
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387