Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HK406_RS18540 Genome accession   NZ_CP053190
Coordinates   3848792..3849772 (-) Length   326 a.a.
NCBI ID   WP_109948520.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain EGYMCRVIM     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3843792..3854772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HK406_RS18510 - 3844669..3845166 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  HK406_RS18515 endA 3845261..3845968 (+) 708 WP_003860034.1 deoxyribonuclease I -
  HK406_RS18520 rsmE 3846020..3846751 (+) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HK406_RS18525 gshB 3846783..3847730 (+) 948 WP_003860032.1 glutathione synthase -
  HK406_RS18530 - 3847805..3848365 (+) 561 WP_003860031.1 YqgE/AlgH family protein -
  HK406_RS18535 ruvX 3848365..3848781 (+) 417 WP_003860030.1 Holliday junction resolvase RuvX -
  HK406_RS18540 pilT 3848792..3849772 (-) 981 WP_109948520.1 type IV pilus twitching motility protein PilT Machinery gene
  HK406_RS18545 - 3849790..3850491 (+) 702 WP_047053922.1 YggS family pyridoxal phosphate-dependent enzyme -
  HK406_RS18550 - 3850513..3851079 (+) 567 WP_003860024.1 YggT family protein -
  HK406_RS18555 yggU 3851076..3851372 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  HK406_RS18560 - 3851376..3851969 (+) 594 WP_003860021.1 XTP/dITP diphosphatase -
  HK406_RS18565 hemW 3851962..3853110 (+) 1149 WP_045332868.1 radical SAM family heme chaperone HemW -
  HK406_RS18570 - 3853167..3853529 (+) 363 WP_022651832.1 DUF559 domain-containing protein -
  HK406_RS18575 - 3853596..3854312 (-) 717 WP_003862419.1 DUF2884 domain-containing protein -
  HK406_RS18580 - 3854370..3854696 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35665.63 Da        Isoelectric Point: 6.4131

>NTDB_id=443408 HK406_RS18540 WP_109948520.1 3848792..3849772(-) (pilT) [Enterobacter hormaechei strain EGYMCRVIM]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIRQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=443408 HK406_RS18540 WP_109948520.1 3848792..3849772(-) (pilT) [Enterobacter hormaechei strain EGYMCRVIM]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAATG
ACGAACAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCATTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACTCTGGCCGCGATGGTCGATTTTCTCAATCACCAGACGGACGGGCACATTCTGACCCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTCATCCGGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGATACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTTCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTGCTGGTGAATACCTCGGCGGCGGCAAACTTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377