Detailed information NTG0443192

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IUJ45_RS06490
• Genome accession: NZ_CP064367
• Coordinates: 1272701..1273927 (-)
• Length: 408 a.a.
• NCBI ID: WP_002218483.1
• Uniprot ID: Q9K1N4
• Organism: Neisseria meningitidis strain PartJ-Nmeningitidis-RM8376
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1267701..1278927

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IUJ45_RS06480 (IUJ45_06575)	-	1267701..1268708 (+)	1008	Protein_1274	IS5 family transposase	-
IUJ45_RS06485 (IUJ45_06580)	yccS	1270265..1272415 (-)	2151	WP_082308663.1	YccS family putative transporter	-
IUJ45_RS06490 (IUJ45_06585)	pilU	1272701..1273927 (-)	1227	WP_002218483.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IUJ45_RS06495 (IUJ45_06590)	pilT	1274090..1275127 (-)	1038	WP_226893076.1	type IV pilus twitching motility protein PilT	Machinery gene
IUJ45_RS06500 (IUJ45_06595)	-	1275253..1275939 (+)	687	WP_002239404.1	YggS family pyridoxal phosphate-dependent enzyme	-
IUJ45_RS06505 (IUJ45_06600)	-	1275939..1276346 (+)	408	WP_002239400.1	hypothetical protein	-
IUJ45_RS06510 (IUJ45_06605)	proC	1276395..1277186 (+)	792	WP_105182941.1	pyrroline-5-carboxylate reductase	-
IUJ45_RS06515 (IUJ45_06610)	dksA	1277334..1277750 (+)	417	WP_002231430.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45674.28 Da
• Isoelectric Point: 6.9640

>NTDB_id=443192 IUJ45_RS06490 WP_002218483.1 1272701..1273927(-) (pilU) [Neisseria meningitidis strain PartJ-Nmeningitidis-RM8376]
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=443192 IUJ45_RS06490 WP_002218483.1 1272701..1273927(-) (pilU) [Neisseria meningitidis strain PartJ-Nmeningitidis-RM8376]
ATAAATACCGATAACCTGCACGACATCTTGGACGAAATGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCGCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAGTCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACCACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCAGGAATTGGGCCTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCAGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCCGACGTGGTCTGCATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATCGACATCGCCC
TCAACCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAACAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGATCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: Q9K1N4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.598	88.48	0.395
pilU	Pseudomonas stutzeri DSM 10701	44.663	87.255	0.39
pilU	Vibrio cholerae strain A1552	46.429	82.353	0.382