Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   IRJ28_RS05270 Genome accession   NZ_CP064096
Coordinates   998206..998823 (+) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   P39694
Organism   Bacillus subtilis strain N1142-3at     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 993206..1003823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ28_RS05235 aroE 993864..994706 (+) 843 WP_003229967.1 shikimate dehydrogenase -
  IRJ28_RS05240 yhbY 994700..994990 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  IRJ28_RS05245 nadD 995002..995571 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  IRJ28_RS05250 yqeK 995561..996121 (+) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  IRJ28_RS05255 rsfS 996139..996495 (+) 357 WP_003229971.1 ribosome silencing factor -
  IRJ28_RS05260 yqeM 996492..997235 (+) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  IRJ28_RS05265 comER 997301..998122 (-) 822 WP_004398597.1 late competence protein ComER -
  IRJ28_RS05270 comEA 998206..998823 (+) 618 WP_004398514.1 competence protein ComEA Machinery gene
  IRJ28_RS05275 comEB 998890..999459 (+) 570 WP_003229978.1 ComE operon protein 2 -
  IRJ28_RS05280 comEC 999463..1001793 (+) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  IRJ28_RS05285 yqzM 1001833..1001967 (-) 135 WP_003229983.1 YqzM family protein -
  IRJ28_RS05290 - 1002008..1002157 (+) 150 WP_003229985.1 hypothetical protein -
  IRJ28_RS05295 holA 1002197..1003240 (+) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  IRJ28_RS05300 rpsT 1003255..1003521 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=442166 IRJ28_RS05270 WP_004398514.1 998206..998823(+) (comEA) [Bacillus subtilis strain N1142-3at]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=442166 IRJ28_RS05270 WP_004398514.1 998206..998823(+) (comEA) [Bacillus subtilis strain N1142-3at]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8DFK

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39