Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   IRJ27_RS18220 Genome accession   NZ_CP064095
Coordinates   3533842..3534459 (+) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   P39694
Organism   Bacillus subtilis strain N1303-2Ay     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3528842..3539459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ27_RS18185 aroE 3529500..3530342 (+) 843 WP_003229967.1 shikimate dehydrogenase -
  IRJ27_RS18190 yhbY 3530336..3530626 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  IRJ27_RS18195 nadD 3530638..3531207 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  IRJ27_RS18200 yqeK 3531197..3531757 (+) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  IRJ27_RS18205 rsfS 3531775..3532131 (+) 357 WP_003229971.1 ribosome silencing factor -
  IRJ27_RS18210 yqeM 3532128..3532871 (+) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  IRJ27_RS18215 comER 3532937..3533758 (-) 822 WP_004398597.1 late competence protein ComER -
  IRJ27_RS18220 comEA 3533842..3534459 (+) 618 WP_004398514.1 competence protein ComEA Machinery gene
  IRJ27_RS18225 comEB 3534526..3535095 (+) 570 WP_003229978.1 ComE operon protein 2 -
  IRJ27_RS18230 comEC 3535099..3537429 (+) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  IRJ27_RS18235 yqzM 3537469..3537603 (-) 135 WP_003229983.1 YqzM family protein -
  IRJ27_RS18240 - 3537644..3537793 (+) 150 WP_003229985.1 hypothetical protein -
  IRJ27_RS18245 holA 3537833..3538876 (+) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  IRJ27_RS18250 rpsT 3538891..3539157 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=442111 IRJ27_RS18220 WP_004398514.1 3533842..3534459(+) (comEA) [Bacillus subtilis strain N1303-2Ay]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=442111 IRJ27_RS18220 WP_004398514.1 3533842..3534459(+) (comEA) [Bacillus subtilis strain N1303-2Ay]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8DFK

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39