Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H9K44_RS03145 Genome accession   NZ_CP064035
Coordinates   647376..647834 (-) Length   152 a.a.
NCBI ID   WP_169608820.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain DLM1799     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 642376..652834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9K44_RS03125 (H9K44_03130) coaE 642898..643512 (-) 615 WP_005480887.1 dephospho-CoA kinase -
  H9K44_RS03130 (H9K44_03135) pilD 643513..644382 (-) 870 WP_169608825.1 A24 family peptidase Machinery gene
  H9K44_RS03135 (H9K44_03140) pilC 644447..645670 (-) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  H9K44_RS03140 (H9K44_03145) pilB 645694..647379 (-) 1686 WP_169608822.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H9K44_RS03145 (H9K44_03150) pilA 647376..647834 (-) 459 WP_169608820.1 pilin Machinery gene
  H9K44_RS03150 (H9K44_03155) nadC 648097..648984 (-) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  H9K44_RS03155 (H9K44_03160) ampD 649077..649628 (+) 552 WP_020841256.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  H9K44_RS03160 (H9K44_03165) pdhR 650034..650801 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 16193.62 Da        Isoelectric Point: 9.0377

>NTDB_id=441305 H9K44_RS03145 WP_169608820.1 647376..647834(-) (pilA) [Vibrio parahaemolyticus strain DLM1799]
MKQSKQKKQQGFTLIELMIVVAVIGVLAAIAMPQYQKYVAKSEVASVLATLTGAKTNVEAYTVENGLFPNNVSDASPTAL
GVPDMPLGSVVFKRDDNTDGGTIEFTFTSVTSGASSLVATKNLTLTRTKEKGSWACSSTNLENELLPKTCKK

Nucleotide


Download         Length: 459 bp        

>NTDB_id=441305 H9K44_RS03145 WP_169608820.1 647376..647834(-) (pilA) [Vibrio parahaemolyticus strain DLM1799]
ATGAAACAGAGTAAACAGAAAAAACAGCAAGGTTTTACGCTGATTGAATTAATGATTGTGGTGGCAGTGATTGGTGTCTT
GGCGGCGATTGCAATGCCACAGTACCAAAAATACGTTGCCAAAAGCGAAGTAGCATCTGTTTTAGCCACACTTACTGGTG
CAAAAACTAACGTAGAAGCATACACAGTAGAAAACGGCTTATTTCCAAACAATGTCTCTGACGCATCACCGACGGCTTTG
GGTGTACCAGACATGCCGCTGGGAAGTGTAGTGTTTAAAAGGGATGATAATACTGATGGTGGTACAATAGAATTTACTTT
TACCTCAGTAACTAGCGGAGCAAGCTCCTTAGTAGCGACCAAAAATTTAACACTCACTAGAACAAAAGAAAAAGGGTCTT
GGGCTTGTTCATCTACCAACCTAGAAAATGAACTTCTCCCAAAAACCTGCAAAAAATGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-32)

Pilin (bacterial filament) (Pilin)

(40-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

46.753

100

0.474
  pilA Vibrio cholerae C6706

46.753

100

0.474
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

46.753

100

0.474
  pilA Pseudomonas aeruginosa PAK

42.763

100

0.428
  pilA Acinetobacter baumannii strain A118

42.657

94.079

0.401
  pilA Vibrio parahaemolyticus RIMD 2210633

47.541

80.263

0.382
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.356

96.053

0.368