Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HIR76_RS13445 Genome accession   NZ_CP051860
Coordinates   2583117..2584187 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2578117..2589187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIR76_RS13400 (HIR76_14205) tapA 2578596..2579357 (-) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  HIR76_RS13405 (HIR76_14200) yqzG 2579629..2579955 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  HIR76_RS13410 (HIR76_14195) spoIITA 2579997..2580176 (-) 180 WP_003230176.1 YqzE family protein -
  HIR76_RS13415 (HIR76_14190) comGG 2580247..2580621 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  HIR76_RS13420 (HIR76_14185) comGF 2580622..2581005 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  HIR76_RS13425 (HIR76_14180) comGE 2581031..2581378 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  HIR76_RS13430 (HIR76_14175) comGD 2581362..2581793 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  HIR76_RS13435 (HIR76_14170) comGC 2581783..2582079 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  HIR76_RS13440 (HIR76_14165) comGB 2582093..2583130 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  HIR76_RS13445 (HIR76_14160) comGA 2583117..2584187 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  HIR76_RS13450 (HIR76_14155) corA 2584599..2585552 (-) 954 WP_004399136.1 magnesium transporter CorA -
  HIR76_RS13455 (HIR76_14150) yqhB 2585695..2587023 (+) 1329 WP_009967730.1 hemolysin family protein -
  HIR76_RS13460 (HIR76_14145) rsbRD 2587076..2587912 (-) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -
  HIR76_RS13470 (HIR76_14135) mgsR 2588136..2588516 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  HIR76_RS13475 (HIR76_14130) yqgY 2588748..2588993 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=441069 HIR76_RS13445 WP_004399124.1 2583117..2584187(-) (comGA) [Bacillus subtilis subsp. subtilis str. 168]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=441069 HIR76_RS13445 WP_004399124.1 2583117..2584187(-) (comGA) [Bacillus subtilis subsp. subtilis str. 168]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGTTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1