Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   HIR76_RS13440 Genome accession   NZ_CP051860
Coordinates   2582093..2583130 (-) Length   345 a.a.
NCBI ID   WP_009967727.1    Uniprot ID   A0AAE2SM96
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2577093..2588130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIR76_RS13390 (HIR76_14215) - 2577579..2578068 (-) 490 Protein_2604 GNAT family N-acetyltransferase -
  HIR76_RS13395 (HIR76_14210) sipW 2578040..2578612 (-) 573 WP_003246088.1 signal peptidase I SipW -
  HIR76_RS13400 (HIR76_14205) tapA 2578596..2579357 (-) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  HIR76_RS13405 (HIR76_14200) yqzG 2579629..2579955 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  HIR76_RS13410 (HIR76_14195) spoIITA 2579997..2580176 (-) 180 WP_003230176.1 YqzE family protein -
  HIR76_RS13415 (HIR76_14190) comGG 2580247..2580621 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  HIR76_RS13420 (HIR76_14185) comGF 2580622..2581005 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  HIR76_RS13425 (HIR76_14180) comGE 2581031..2581378 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  HIR76_RS13430 (HIR76_14175) comGD 2581362..2581793 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  HIR76_RS13435 (HIR76_14170) comGC 2581783..2582079 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  HIR76_RS13440 (HIR76_14165) comGB 2582093..2583130 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  HIR76_RS13445 (HIR76_14160) comGA 2583117..2584187 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  HIR76_RS13450 (HIR76_14155) corA 2584599..2585552 (-) 954 WP_004399136.1 magnesium transporter CorA -
  HIR76_RS13455 (HIR76_14150) yqhB 2585695..2587023 (+) 1329 WP_009967730.1 hemolysin family protein -
  HIR76_RS13460 (HIR76_14145) rsbRD 2587076..2587912 (-) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39762.26 Da        Isoelectric Point: 9.6257

>NTDB_id=441068 HIR76_RS13440 WP_009967727.1 2582093..2583130(-) (comGB) [Bacillus subtilis subsp. subtilis str. 168]
MKKIRKVWLLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSLFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=441068 HIR76_RS13440 WP_009967727.1 2582093..2583130(-) (comGB) [Bacillus subtilis subsp. subtilis str. 168]
ATGAAAAAGATTAGAAAAGTCTGGTTGTTAAAGGATCAAGCCAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACACTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCAATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTAAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCTTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

100

93.623

0.936