Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   INP70_RS11800 Genome accession   NZ_CP063420
Coordinates   2608339..2610066 (-) Length   575 a.a.
NCBI ID   WP_001122154.1    Uniprot ID   Q9KUV7
Organism   Vibrio cholerae strain VC_hf7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2603339..2615066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP70_RS11765 - 2603616..2604386 (-) 771 WP_000086308.1 PilW family protein -
  INP70_RS11770 - 2604386..2604955 (-) 570 WP_001144511.1 type II secretion system protein -
  INP70_RS11775 - 2604945..2605457 (-) 513 WP_001249938.1 type II secretion system protein -
  INP70_RS11780 - 2605521..2605982 (-) 462 WP_000828589.1 type II secretion system protein -
  INP70_RS11785 - 2606017..2606571 (-) 555 WP_000738707.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  INP70_RS11790 - 2606652..2607104 (-) 453 WP_032468787.1 hypothetical protein -
  INP70_RS11795 - 2607107..2608330 (-) 1224 WP_000190441.1 type II secretion system F family protein -
  INP70_RS11800 pilB/pilB1 2608339..2610066 (-) 1728 WP_001122154.1 GspE/PulE family protein Machinery gene
  INP70_RS11805 - 2610056..2611207 (-) 1152 WP_000117838.1 lipopolysaccharide assembly protein LapB -
  INP70_RS11810 - 2611204..2612049 (-) 846 WP_000281356.1 ExeA family protein -
  INP70_RS11815 mshL 2612054..2613733 (-) 1680 WP_001227117.1 pilus (MSHA type) biogenesis protein MshL -
  INP70_RS11820 - 2613748..2614065 (-) 318 WP_000252812.1 MSHA biogenesis protein MshK -
  INP70_RS11825 gspM 2614058..2614708 (-) 651 WP_000835144.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63844.29 Da        Isoelectric Point: 6.0455

>NTDB_id=439475 INP70_RS11800 WP_001122154.1 2608339..2610066(-) (pilB/pilB1) [Vibrio cholerae strain VC_hf7]
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRAHVDIDAVP
LLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNQLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHA
EHKTNDSFDFEITDSDSDEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMIL
VTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRINQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETV
EIGLRAALTGHLVLSTLHTNDAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQ
AAAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMS
LGEGDSSGLVEPIYL

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=439475 INP70_RS11800 WP_001122154.1 2608339..2610066(-) (pilB/pilB1) [Vibrio cholerae strain VC_hf7]
GTGCCAATTAATAAACTGCGTAAACGGCTTGGTGACTTGCTGGTTGAAGAGGGGATTGTGAGCGAAGCGCAGCTTGAACA
AGCGCTCAATGCCCAGAAAAATACCGGACGCCGCTTAGGGGATACCTTAATTTCGCTTGGCTTTTTAAGCGAAACCCAGT
TGCTGAACTTTTTGGCGCAGCAATTGAGTTTACCGGTGATCGATTTGAGCCGCGCTCATGTCGATATTGATGCGGTTCCT
TTACTGCCTGAAGTTCATGCCCGCCGTTTGCGTGCGCTGGTGATAGGCCGCAGTGGTGATACGCTGCGCATAGCCATGAG
TGATCCTGCGGATCTGTTTGCTCAAGAAGCCTTGCTCAACCAGTTGCCTGATTACGGTTTTGAGTTTGTCATCGCTCCTG
AAAAACAGTTGGTCGATGGCTTCGATCGTTACTATCGCCGCACCAAAGAAATTGTTTCGTTCGCCGAACAATTGCATGCC
GAACACAAAACCAATGATAGTTTTGATTTTGAGATCACCGATTCAGATAGCGATGAAGTGACGGTTGTTAAACTGATCAA
CTCGCTGTTTGAAGATGCGATTCAAGTGGGAGCCTCGGATATACACATTGAGCCGGATGCCAACGTACTGCGTTTGCGTC
AACGGATCGATGGGGTACTGCATGAAACCTTGCTCCATGAAGTGAATATCGCCTCAGCCTTAGTGCTGCGCTTAAAATTA
ATGGCGAATCTGGATATTTCAGAAAAACGCCTTCCGCAAGATGGACGTTTTAACATTCGTGTAAAAGGACAATCCGTCGA
TATCCGGATGTCAACCATGCCTATCCAGCACGGTGAATCGGTGGTGATGCGTTTGCTCAACCAATCTGCAGGGGTTAGAA
AGCTCGAAGAGTCGGGTATTCCGCCTCATTTATTGCTGCGTTTGCGCCATCAGTTGAAACGCCCTCATGGCATGATTCTG
GTGACTGGGCCTACCGGCTCGGGGAAAACCACCACCTTGTATGGCGCACTCAACGAGCTCAATACGTCCGGCAAAAAAAT
CATTACTGCGGAAGATCCGGTGGAATACCGGATTTCACGGATCAATCAGGTGCAAGTGAACCCGAAAATCAATCTCGATT
TCTCGACGTTACTGCGCACTTTCTTGCGCCAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCAAGAGACGGTC
GAAATTGGCCTGCGTGCTGCATTAACCGGTCACTTAGTCTTAAGTACTCTGCACACCAATGATGCCGTCGATAGCGCGCT
GCGTTTGATTGATATGGGGGCGCCCGGTTATCTGGTTGCCAGTGCGGTACGTGCCGTGGTTGCGCAGCGTTTAGTGCGAA
AAGTCTGTCCAGATTGTAGTGGACATGATGAGGTTGATGAAGCGCGTCGCCAATGGCTTGTGACGCGTTTCCCTAATCAA
GCTGCCGCCAAATTCACTCGTGGACGCGGCTGCCAAAACTGTAACTTAACCGGTTATCGCGGCCGGATCGGTGTATTTGA
AATGCTTGAGCTGGATCAGCCGATGATGGATTGCTTGCGGGCAAATGATGCTGTGGCCTTCTCTAAAGCCGCACGCAGTA
ACACAGATTATAAACCGCTACTGGCTTCGGCGATGGAGTTAGCGCTGCAAGGTATCGTCAGTCTTGATGAAGTGATGTCG
CTGGGTGAAGGTGATTCCTCTGGTTTGGTTGAACCGATTTATCTGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)

Type II/IV secretion system protein (T2SSE)

(189-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5HTL

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

35.596

100

0.374
  pilB Vibrio campbellii strain DS40M4

36.796

98.783

0.363
  pilB Vibrio cholerae strain A1552

36.945

97.913

0.362
  pilF Thermus thermophilus HB27

37.209

97.217

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.477

96.522

0.362
  pilB Legionella pneumophila strain ERS1305867

39.057

92.174

0.36