Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   HC658_RS12675 Genome accession   NZ_CP051466
Coordinates   2481157..2481774 (-) Length   205 a.a.
NCBI ID   WP_014480312.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain UCMB5021     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2476157..2486774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC658_RS12645 (HC658_25000) rpsT 2476459..2476725 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  HC658_RS12650 (HC658_25010) holA 2476740..2477783 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  HC658_RS12655 - 2477823..2477972 (-) 150 WP_003229985.1 hypothetical protein -
  HC658_RS12660 yqzM 2478013..2478147 (+) 135 WP_003229983.1 YqzM family protein -
  HC658_RS12665 (HC658_25020) comEC 2478187..2480517 (-) 2331 WP_089172590.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  HC658_RS12670 (HC658_25030) comEB 2480521..2481090 (-) 570 WP_003229978.1 ComE operon protein 2 -
  HC658_RS12675 (HC658_25040) comEA 2481157..2481774 (-) 618 WP_014480312.1 competence protein ComEA Machinery gene
  HC658_RS12680 (HC658_25050) comER 2481858..2482679 (+) 822 WP_014480313.1 late competence protein ComER -
  HC658_RS12685 (HC658_25060) yqeM 2482745..2483488 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  HC658_RS12690 (HC658_25070) rsfS 2483485..2483841 (-) 357 WP_014480315.1 ribosome silencing factor -
  HC658_RS12695 (HC658_25080) yqeK 2483859..2484419 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  HC658_RS12700 (HC658_25090) nadD 2484409..2484978 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  HC658_RS12705 (HC658_25100) yhbY 2484990..2485280 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  HC658_RS12710 (HC658_25110) aroE 2485274..2486116 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21781.51 Da        Isoelectric Point: 4.7220

>NTDB_id=438665 HC658_RS12675 WP_014480312.1 2481157..2481774(-) (comEA) [Bacillus subtilis subsp. subtilis strain UCMB5021]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=438665 HC658_RS12675 WP_014480312.1 2481157..2481774(-) (comEA) [Bacillus subtilis subsp. subtilis strain UCMB5021]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39