Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HC658_RS12225 Genome accession   NZ_CP051466
Coordinates   2399688..2400758 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis subsp. subtilis strain UCMB5021     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2394688..2405758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC658_RS12180 (HC658_24040) tapA 2395166..2395927 (-) 762 WP_029726724.1 amyloid fiber anchoring/assembly protein TapA -
  HC658_RS12185 (HC658_24050) yqzG 2396199..2396525 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  HC658_RS12190 (HC658_24060) spoIITA 2396567..2396746 (-) 180 WP_029726723.1 YqzE family protein -
  HC658_RS12195 (HC658_24070) comGG 2396818..2397192 (-) 375 WP_029726722.1 ComG operon protein ComGG Machinery gene
  HC658_RS12200 (HC658_24080) comGF 2397193..2397576 (-) 384 WP_032722118.1 ComG operon protein ComGF Machinery gene
  HC658_RS12205 (HC658_24090) comGE 2397602..2397949 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  HC658_RS12210 (HC658_24100) comGD 2397933..2398364 (-) 432 WP_029726720.1 comG operon protein ComGD Machinery gene
  HC658_RS12215 (HC658_24110) comGC 2398354..2398650 (-) 297 WP_029726719.1 comG operon protein ComGC Machinery gene
  HC658_RS12220 (HC658_24120) comGB 2398664..2399701 (-) 1038 WP_029317912.1 comG operon protein ComGB Machinery gene
  HC658_RS12225 (HC658_24130) comGA 2399688..2400758 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  HC658_RS12230 (HC658_24150) - 2400971..2401168 (-) 198 WP_014480259.1 CBS domain-containing protein -
  HC658_RS12235 (HC658_24160) corA 2401170..2402123 (-) 954 WP_004399136.1 magnesium transporter CorA -
  HC658_RS12240 (HC658_24170) yqhB 2402266..2403594 (+) 1329 WP_003230150.1 hemolysin family protein -
  HC658_RS12245 (HC658_24180) rsbRD 2403647..2404483 (-) 837 WP_003230149.1 RsbT co-antagonist protein RsbRD -
  HC658_RS12255 (HC658_24200) mgsR 2404707..2405087 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  HC658_RS12260 (HC658_24210) yqgY 2405319..2405564 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=438661 HC658_RS12225 WP_004399124.1 2399688..2400758(-) (comGA) [Bacillus subtilis subsp. subtilis strain UCMB5021]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=438661 HC658_RS12225 WP_004399124.1 2399688..2400758(-) (comGA) [Bacillus subtilis subsp. subtilis strain UCMB5021]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGTCGCCAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGGAAAGGAATTGCGCTCGGCTATTTAACAACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1