Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   HC659_RS12635 Genome accession   NZ_CP051465
Coordinates   2480945..2481562 (-) Length   205 a.a.
NCBI ID   WP_014480312.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain UCMB5121     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2475945..2486562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC659_RS12605 (HC659_24960) rpsT 2476247..2476513 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  HC659_RS12610 (HC659_24970) holA 2476528..2477571 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  HC659_RS12615 - 2477611..2477760 (-) 150 WP_003229985.1 hypothetical protein -
  HC659_RS12620 yqzM 2477801..2477935 (+) 135 WP_003229983.1 YqzM family protein -
  HC659_RS12625 (HC659_24980) comEC 2477975..2480305 (-) 2331 WP_089172590.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  HC659_RS12630 (HC659_24990) comEB 2480309..2480878 (-) 570 WP_003229978.1 ComE operon protein 2 -
  HC659_RS12635 (HC659_25000) comEA 2480945..2481562 (-) 618 WP_014480312.1 competence protein ComEA Machinery gene
  HC659_RS12640 (HC659_25010) comER 2481646..2482467 (+) 822 WP_014480313.1 late competence protein ComER -
  HC659_RS12645 (HC659_25020) yqeM 2482533..2483276 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  HC659_RS12650 (HC659_25030) rsfS 2483273..2483629 (-) 357 WP_014480315.1 ribosome silencing factor -
  HC659_RS12655 (HC659_25040) yqeK 2483647..2484207 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  HC659_RS12660 (HC659_25050) nadD 2484197..2484766 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  HC659_RS12665 (HC659_25060) yhbY 2484778..2485068 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  HC659_RS12670 (HC659_25070) aroE 2485062..2485904 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21781.51 Da        Isoelectric Point: 4.7220

>NTDB_id=438582 HC659_RS12635 WP_014480312.1 2480945..2481562(-) (comEA) [Bacillus subtilis subsp. subtilis strain UCMB5121]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=438582 HC659_RS12635 WP_014480312.1 2480945..2481562(-) (comEA) [Bacillus subtilis subsp. subtilis strain UCMB5121]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39