Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   IN878_RS10670 Genome accession   NZ_CP063332
Coordinates   2170382..2171041 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain BS74R-D     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2165382..2176041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IN878_RS10645 (IN878_10655) ygiS 2166665..2168272 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  IN878_RS10650 (IN878_10660) mqsA 2168405..2168800 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  IN878_RS10655 (IN878_10665) mqsR 2168802..2169098 (-) 297 Protein_2120 type II toxin-antitoxin system toxin MqsR -
  IN878_RS10660 (IN878_10670) ygiV 2169303..2169785 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  IN878_RS10665 (IN878_10675) ygiW 2169838..2170230 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  IN878_RS10670 (IN878_10680) ciaR 2170382..2171041 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  IN878_RS10675 (IN878_10685) qseC 2171038..2172387 (+) 1350 WP_203037215.1 quorum sensing histidine kinase QseC -
  IN878_RS10680 (IN878_10690) ygiZ 2172433..2172765 (-) 333 WP_000917684.1 DUF2645 family protein -
  IN878_RS10685 (IN878_10695) mdaB 2173084..2173665 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  IN878_RS10690 (IN878_10700) ygiN 2173696..2174010 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  IN878_RS10695 (IN878_10705) parE 2174058..2175950 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=437835 IN878_RS10670 WP_001221493.1 2170382..2171041(+) (ciaR) [Escherichia coli strain BS74R-D]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=437835 IN878_RS10670 WP_001221493.1 2170382..2171041(+) (ciaR) [Escherichia coli strain BS74R-D]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365