Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSF113_RS55815 Genome accession   NC_016830
Coordinates   5807898..5809598 (+) Length   566 a.a.
NCBI ID   WP_014340251.1    Uniprot ID   -
Organism   Pseudomonas ogarae     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5802898..5814598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSF113_RS55795 (PSF113_5000) - 5803075..5806008 (+) 2934 WP_014340248.1 DUF748 domain-containing protein -
  PSF113_RS55800 (PSF113_5001) - 5806010..5806300 (+) 291 WP_014340249.1 DUF2845 domain-containing protein -
  PSF113_RS55805 (PSF113_5002) - 5806629..5806982 (-) 354 WP_003205664.1 BON domain-containing protein -
  PSF113_RS55810 (PSF113_5003) pilA 5807254..5807673 (-) 420 WP_014340250.1 pilin Machinery gene
  PSF113_RS55815 (PSF113_5004) pilB 5807898..5809598 (+) 1701 WP_014340251.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSF113_RS55820 (PSF113_5005) pilC 5809601..5810818 (+) 1218 WP_014340252.1 type II secretion system F family protein Machinery gene
  PSF113_RS55825 (PSF113_5006) pilD 5810820..5811689 (+) 870 WP_041476149.1 A24 family peptidase Machinery gene
  PSF113_RS55830 (PSF113_5007) coaE 5811689..5812312 (+) 624 WP_041476150.1 dephospho-CoA kinase -
  PSF113_RS55835 (PSF113_5008) yacG 5812309..5812509 (+) 201 WP_041476151.1 DNA gyrase inhibitor YacG -
  PSF113_RS55840 (PSF113_5009) - 5812506..5812721 (-) 216 WP_013694234.1 hypothetical protein -
  PSF113_RS55845 (PSF113_5010) - 5812785..5813471 (-) 687 WP_014340256.1 energy-coupling factor ABC transporter permease -
  PSF113_RS55850 (PSF113_5011) - 5813513..5813923 (-) 411 WP_014340257.1 tautomerase family protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62714.74 Da        Isoelectric Point: 5.4372

>NTDB_id=43750 PSF113_RS55815 WP_014340251.1 5807898..5809598(+) (pilB) [Pseudomonas ogarae]
MNDIALSGLTKQLVLAELLTEQSAQQAYQQAQRSRIPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKETQPTGL
VSEKLVRQHHALPLWRRGNKLYVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDDTRQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPIQLANRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKNLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCSHCKKAIEIPEETLLKEGFPR
ERIGTFTIYEPVGCEQCNNGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRASGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=43750 PSF113_RS55815 WP_014340251.1 5807898..5809598(+) (pilB) [Pseudomonas ogarae]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGCTGGCCGAACTGCTCACCGAGCAAAGTGCGCAACAGGC
GTATCAACAGGCCCAACGCAGTCGCATTCCCCTGGTCAGCTACTTGGTGCAGAACAAACTGGTCCAGAGCCGCCAGGTCG
CGGAGATCGCCTCGGAGCATTTCGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAAGAAACCCAACCCACCGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCACCACGCCCTGCCGCTGTGGCGGCGTGGCAACAAGCTGTACGTGGGCATCTCCGA
CCCCACCAATCACCAGGCCATCAACGACATCCAGTTCAGCACTGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAGTTTTTCGAGTCCAGCAGCACCGGCCTGGAAGGCATGGGCGATGTCGACCTCGACGGC
CTGGACATCGAATCGATCGACGACACCAGGCAGGACTCCATCGCCGGCCAGGACACCGACGACGCACCTGTGGTGCGCTT
CGTCAACAAGATGCTGCTGGACGCAATCAAGGGTGGCTCTTCCGACTTGCACTTCGAGCCCTATGAAAAGATCTACCGGG
TACGGGTGCGCACCGACGGCATGCTGCGAGAAGTGGCCAGGCCGCCGATCCAACTGGCCAACCGTATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGATATTTCCGAGCGGCGCAAACCCCAGGACGGGCGCCTGAAAATGCGCCTGTCAAAAACCAA
GTCCATCGATTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CGCAAATGGGCATCGATGCCCTGGGCTACGAACCGGACCAGAAAAACCTGTACATGGCCGCCCTCAAGCAACCCCAAGGG
CTGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGACTGAATATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTGAATCCGCGCCAGG
GGCTGGATTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCAGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGCCTGCAGAACATGGGCATTCCGGGGTTCAATATCGCCACCGCCGTGCACCTGATCATTGCCCAGCGGC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGAAGGCCATCGAGATTCCCGAGGAAACCTTGCTCAAGGAAGGTTTCCCTCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAATAACGGCTACAAAGGGCGCGTGGGGGT
TTACGAAGTGGTCAAGAACACGCCCGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATCTGCAGA
TGCGCAAGGACGGCTTCAATGACCTGCGCGCATCGGGGCTGCTCAAAGTGATGCAGGGCGTCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.304

100

0.583
  pilB Acinetobacter baumannii D1279779

57.848

100

0.58
  pilB Legionella pneumophila strain ERS1305867

55.379

100

0.555
  pilB Vibrio cholerae strain A1552

50.442

99.823

0.504
  pilB Vibrio campbellii strain DS40M4

48.596

100

0.489
  pilF Neisseria gonorrhoeae MS11

48.313

99.47

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

48.921

98.233

0.481
  pilF Thermus thermophilus HB27

36.042

100

0.36

Multiple sequence alignment