Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HEP75_RS18430 Genome accession   NZ_CP051261
Coordinates   4360640..4361788 (+) Length   382 a.a.
NCBI ID   WP_185814023.1    Uniprot ID   A0A7G7T172
Organism   Xanthomonas sp. SI     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4355640..4366788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HEP75_RS18405 (HEP75_03619) - 4355841..4357037 (-) 1197 WP_185814020.1 cystathionine gamma-synthase -
  HEP75_RS18410 (HEP75_03620) - 4357120..4358490 (-) 1371 WP_185824474.1 pyridoxal-phosphate dependent enzyme -
  HEP75_RS18415 (HEP75_03621) - 4358693..4358917 (-) 225 WP_009575526.1 YdcH family protein -
  HEP75_RS18420 (HEP75_03622) - 4359255..4360133 (-) 879 WP_185816693.1 OmpA family protein -
  HEP75_RS18425 (HEP75_03623) - 4360148..4360540 (-) 393 WP_185824475.1 DUF4398 domain-containing protein -
  HEP75_RS18430 (HEP75_03624) pilU 4360640..4361788 (+) 1149 WP_185814023.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HEP75_RS18435 (HEP75_03625) maiA 4361890..4362549 (-) 660 WP_185824476.1 maleylacetoacetate isomerase -
  HEP75_RS18440 (HEP75_03626) - 4362639..4363628 (-) 990 WP_185824477.1 fumarylacetoacetate hydrolase family protein -
  HEP75_RS18445 (HEP75_03627) - 4364005..4365315 (+) 1311 Protein_3627 DEAD/DEAH box helicase -
  HEP75_RS18450 (HEP75_03628) queD 4365495..4365854 (+) 360 WP_185814027.1 6-carboxytetrahydropterin synthase QueD -
  HEP75_RS18455 (HEP75_03629) - 4366135..4366506 (+) 372 WP_185824479.1 phasin family protein -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42245.53 Da        Isoelectric Point: 6.4456

>NTDB_id=437144 HEP75_RS18430 WP_185814023.1 4360640..4361788(+) (pilU) [Xanthomonas sp. SI]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGATGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALQDAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELHLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKDKNERRRPATEVLLNTPMIRDLLRRGQVHEIKAAMEESLEEGMETFDQCL
FRMVKQGQIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADYDAGGSGGASSPRITHGFG

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=437144 HEP75_RS18430 WP_185814023.1 4360640..4361788(+) (pilU) [Xanthomonas sp. SI]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCCTCGGACATGTTCCTGACCACGGGAGCACCGGTCTA
TATCAAGATCGAAGGCAAGCTGTACCCGCTCGGCGCCACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTACCGCAGTTCGAGCGCGAGCTGGAGCTGAACATGGCCATCGCGCTGCAGGACGCCGGCCGC
TTCCGGGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGCGCGATCCGCAGCAAGATCCCCAGCATCGA
GGAACTGCACCTGCCGCAGGTGCTCAAGGACGTGATCATGACCCCGCGCGGGCTGGTCCTGGTGGTCGGCTCCACCGGCT
CGGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGACGACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGATCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGGTCTCGCAGCG
CCTGGTCAAGGACAAGAACGAGCGCCGCCGCCCGGCCACCGAGGTGCTGCTGAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTCCACGAGATCAAGGCGGCCATGGAGGAATCGCTGGAGGAAGGCATGGAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAACAGGGCCAGATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGCCTATCCGAAGGCTCCAGCGGCGAACACGACCCTTACGCCGACTACGACGCCGGCGGCAGCGGCGGCGCCAGCT
CGCCGCGGATCACCCACGGCTTCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(47-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G7T172

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

93.979

0.482
  pilU Vibrio cholerae strain A1552

47.619

98.953

0.471
  pilU Acinetobacter baylyi ADP1

49.153

92.67

0.456
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.531

93.717

0.427
  pilT Pseudomonas aeruginosa PAK

42.012

88.482

0.372
  pilT Acinetobacter baumannii D1279779

40.828

88.482

0.361
  pilT Acinetobacter baumannii strain A118

40.828

88.482

0.361
  pilT Acinetobacter nosocomialis M2

40.828

88.482

0.361