Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HF584_RS01490 Genome accession   NZ_CP051165
Coordinates   305686..306660 (+) Length   324 a.a.
NCBI ID   WP_054191886.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NGA84b     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 300686..311660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF584_RS01455 (HF584_07400) rpmG 301146..301295 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  HF584_RS01460 (HF584_07395) - 301504..302043 (+) 540 WP_077446656.1 5-formyltetrahydrofolate cyclo-ligase -
  HF584_RS01465 (HF584_07390) - 302055..303518 (+) 1464 WP_054190029.1 rhomboid family intramembrane serine protease -
  HF584_RS01470 (HF584_07385) - 303499..303702 (+) 204 WP_000087561.1 YqgQ family protein -
  HF584_RS01475 (HF584_07380) - 303699..304685 (+) 987 WP_000161315.1 ROK family glucokinase -
  HF584_RS01480 (HF584_07375) - 304685..305014 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  HF584_RS01485 (HF584_07370) - 305011..305634 (+) 624 WP_054190028.1 MBL fold metallo-hydrolase -
  HF584_RS01490 (HF584_07365) comGA 305686..306660 (+) 975 WP_054191886.1 competence type IV pilus ATPase ComGA Machinery gene
  HF584_RS01495 (HF584_07360) comGB 306632..307702 (+) 1071 WP_054190027.1 competence type IV pilus assembly protein ComGB Machinery gene
  HF584_RS01500 (HF584_07355) comGC 307716..308027 (+) 312 WP_054190026.1 competence type IV pilus major pilin ComGC Machinery gene
  HF584_RS01505 (HF584_07350) comGD 308005..308451 (+) 447 WP_077446655.1 competence type IV pilus minor pilin ComGD Machinery gene
  HF584_RS01510 (HF584_07345) comGE 308438..308737 (+) 300 WP_054190024.1 hypothetical protein Machinery gene
  HF584_RS01515 (HF584_07340) comGF 308655..309152 (+) 498 WP_077446653.1 competence type IV pilus minor pilin ComGF Machinery gene
  HF584_RS01520 (HF584_07335) - 309249..309395 (+) 147 WP_001789879.1 hypothetical protein -
  HF584_RS01525 (HF584_07330) - 309385..309909 (+) 525 WP_054190023.1 shikimate kinase -
  HF584_RS01530 (HF584_07325) gcvT 310068..311159 (+) 1092 WP_054190022.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36912.97 Da        Isoelectric Point: 8.6529

>NTDB_id=436440 HF584_RS01490 WP_054191886.1 305686..306660(+) (comGA) [Staphylococcus aureus strain NGA84b]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLIYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHDHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=436440 HF584_RS01490 WP_054191886.1 305686..306660(+) (comGA) [Staphylococcus aureus strain NGA84b]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAATTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAAAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGTCCAACTGGTTCAGGTAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAGGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAGGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATGATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.383

100

0.994
  comGA Staphylococcus aureus N315

99.383

100

0.994