Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   HF584_RS01495 Genome accession   NZ_CP051165
Coordinates   306632..307702 (+) Length   356 a.a.
NCBI ID   WP_054190027.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NGA84b     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 301632..312702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF584_RS01465 (HF584_07390) - 302055..303518 (+) 1464 WP_054190029.1 rhomboid family intramembrane serine protease -
  HF584_RS01470 (HF584_07385) - 303499..303702 (+) 204 WP_000087561.1 YqgQ family protein -
  HF584_RS01475 (HF584_07380) - 303699..304685 (+) 987 WP_000161315.1 ROK family glucokinase -
  HF584_RS01480 (HF584_07375) - 304685..305014 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  HF584_RS01485 (HF584_07370) - 305011..305634 (+) 624 WP_054190028.1 MBL fold metallo-hydrolase -
  HF584_RS01490 (HF584_07365) comGA 305686..306660 (+) 975 WP_054191886.1 competence type IV pilus ATPase ComGA Machinery gene
  HF584_RS01495 (HF584_07360) comGB 306632..307702 (+) 1071 WP_054190027.1 competence type IV pilus assembly protein ComGB Machinery gene
  HF584_RS01500 (HF584_07355) comGC 307716..308027 (+) 312 WP_054190026.1 competence type IV pilus major pilin ComGC Machinery gene
  HF584_RS01505 (HF584_07350) comGD 308005..308451 (+) 447 WP_077446655.1 competence type IV pilus minor pilin ComGD Machinery gene
  HF584_RS01510 (HF584_07345) comGE 308438..308737 (+) 300 WP_054190024.1 hypothetical protein Machinery gene
  HF584_RS01515 (HF584_07340) comGF 308655..309152 (+) 498 WP_077446653.1 competence type IV pilus minor pilin ComGF Machinery gene
  HF584_RS01520 (HF584_07335) - 309249..309395 (+) 147 WP_001789879.1 hypothetical protein -
  HF584_RS01525 (HF584_07330) - 309385..309909 (+) 525 WP_054190023.1 shikimate kinase -
  HF584_RS01530 (HF584_07325) gcvT 310068..311159 (+) 1092 WP_054190022.1 glycine cleavage system aminomethyltransferase GcvT -
  HF584_RS01535 (HF584_07320) gcvPA 311179..312525 (+) 1347 WP_054190021.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41579.02 Da        Isoelectric Point: 10.1371

>NTDB_id=436441 HF584_RS01495 WP_054190027.1 306632..307702(+) (comGB) [Staphylococcus aureus strain NGA84b]
MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLATTILSEISNGAPCNQILSLIG
YNDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSIFITSLPTIIVVMLIIVSMLAIIMKFIYNKLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKFLLTYSEMGYSLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVRQIEDKAIK
QTQFLQPILFFILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=436441 HF584_RS01495 WP_054190027.1 306632..307702(+) (comGB) [Staphylococcus aureus strain NGA84b]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGCTACCACCATTCTGAGTGAAATTTCAAATGGTGCACCATGCAACCAGATATTATCACTGATAGGT
TATAACGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATCATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATTTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTATTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTTATTTATAACAAATTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTTATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTTTAGGTAAATTCTTATTAACTTATTCGGA
AATGGGATATAGTTTACCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATAAAGTTTGTGCTGCAAGGTG
AAAAGAGAGGGAAACTAGAAGTAGAACTAAAGTTATATTCACAAATATTAGTGAGACAAATAGAGGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTTATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

97.472

100

0.975
  comGB Staphylococcus aureus N315

97.472

100

0.975