Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IL983_RS22505 Genome accession   NZ_CP063047
Coordinates   4904964..4905587 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain KC-Tt-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4899964..4910587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IL983_RS22485 (IL983_22485) aroB 4900559..4901665 (-) 1107 WP_009314395.1 3-dehydroquinate synthase -
  IL983_RS22490 (IL983_22490) aroK 4901715..4902233 (-) 519 WP_003095833.1 shikimate kinase AroK -
  IL983_RS22495 (IL983_22495) pilQ 4902245..4904389 (-) 2145 WP_003103267.1 type 4a pilus secretin PilQ Machinery gene
  IL983_RS22500 (IL983_22500) comL/pilP 4904443..4904967 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  IL983_RS22505 (IL983_22505) pilO 4904964..4905587 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  IL983_RS22510 (IL983_22510) pilN 4905584..4906180 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  IL983_RS22515 (IL983_22515) pilM 4906180..4907244 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  IL983_RS22520 (IL983_22520) - 4907429..4909900 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=436259 IL983_RS22505 WP_003103268.1 4904964..4905587(-) (pilO) [Pseudomonas aeruginosa strain KC-Tt-1]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=436259 IL983_RS22505 WP_003103268.1 4904964..4905587(-) (pilO) [Pseudomonas aeruginosa strain KC-Tt-1]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAACCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401