Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GHB95_RS00490 Genome accession   NZ_CP050930
Coordinates   101393..103588 (-) Length   731 a.a.
NCBI ID   WP_003696681.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain NG211     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 96393..108588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHB95_RS00470 (GHB95_00465) - 96434..96898 (-) 465 WP_003687344.1 transcriptional regulator NrdR -
  GHB95_RS00475 (GHB95_00470) - 96928..97770 (-) 843 WP_003687345.1 class II glutamine amidotransferase -
  GHB95_RS00480 (GHB95_00475) aroB 98509..99588 (-) 1080 WP_003690567.1 3-dehydroquinate synthase -
  GHB95_RS00485 (GHB95_00480) - 99668..100180 (-) 513 WP_003687349.1 shikimate kinase -
  GHB95_RS00490 (GHB95_00485) pilQ 101393..103588 (-) 2196 WP_003696681.1 type IV pilus secretin PilQ Machinery gene
  GHB95_RS00495 (GHB95_00490) pilP 103607..104152 (-) 546 WP_003687351.1 pilus assembly protein PilP Machinery gene
  GHB95_RS00500 (GHB95_00495) pilO 104170..104817 (-) 648 WP_003690570.1 type 4a pilus biogenesis protein PilO -
  GHB95_RS00505 (GHB95_00500) pilN 104818..105417 (-) 600 WP_003687354.1 PilN domain-containing protein Machinery gene
  GHB95_RS00510 (GHB95_00505) - 105420..106535 (-) 1116 WP_003687356.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 731 a.a.        Molecular weight: 78746.83 Da        Isoelectric Point: 10.0442

>NTDB_id=435671 GHB95_RS00490 WP_003696681.1 101393..103588(-) (pilQ) [Neisseria gonorrhoeae strain NG211]
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVL
EYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV
SVSAPFSPAKQQAAASAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTALQRSLD
VADFKTPVQKVTLKRLNNDTQLIITTTGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIR
TILQILAKESGMNIVASDSVSGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADL
GALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMI
EARIVEAADGFSRDLGVKFGATGRKKLKNETSAFGWGVNSGFGGGDKWEAQTKINLPVAAAANSISLVRAISSGALNLEL
SASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTTASGGGNSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDS
PAQCASGNNTILCISTKSLNTQAMVENGGTLIVGGIYEENNGNTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPR
IIDTAGNSLRY

Nucleotide


Download         Length: 2196 bp        

>NTDB_id=435671 GHB95_RS00490 WP_003696681.1 101393..103588(-) (pilQ) [Neisseria gonorrhoeae strain NG211]
ATGAATACCAAACTGACAAAAATCATTTCCGGTCTCTTTGTCGCAACCGCCGCCTTTCAGACGGCATCGGCAGGAAACAT
TACAGACATCAAAGTTTCCTCCCTGCCCAACAAACAGAAAATCGTCAAAGTCAGCTTTGACAAAGAGATTGTCAACCCGA
CCGGCTTCGTAACCTCCTCACCGGCCCGCATCGCCTTGGACTTTGAACAAACCGGCATTTCCATGGATCAACAGGTACTC
GAATATGCCGATCCTCTGTTGAGCAAAATCAGTGCCGCACAAAACAGCAGCCGTGCGCGTCTGGTTCTGAATTTGAACAA
ACCGGGCCAATACAATACCGAAGTACGCGGGAACAAAGTTTGGATATTCATTAACGAATCGGACGATACCGTGTCCGCCC
CCGCCCGCCCAGCCGTAAAAGCCGCGCCTGCCGCACCGGCAAAACAACAGGCTGCCGCACCGTTTACCGAGTCCGTAGTA
TCCGTATCCGCACCGTTCAGCCCGGCAAAACAACAGGCAGCGGCATCGGCAAAACAACAGGCGGCGACACCGGCAAAACA
AACCAATATCGATTTCCGCAAAGACGGCAAAAATGCCGGCATTATCGAATTGGCGGCATTGGGCTTTGCCGGGCAGCCCG
ACATCAGCCAACAGCACGACCACATCATCGTTACGCTGAAAAACCATACCCTGCCGACCGCGCTCCAACGCAGTTTGGAT
GTGGCAGACTTCAAAACACCGGTTCAAAAGGTTACGCTGAAACGCCTCAATAACGACACCCAGCTGATTATCACAACAAC
CGGCAACTGGGAACTCGTCAACAAATCCGCCGCGCCCGGATACTTTACCTTCCAAGTCCTGCCGAAAAAACAAAACCTCG
AGTCAGGCGGCGTGAACAATGCGCCCAAAACCTTCACAGGCCGGAAAATCTCCCTTGACTTCCAAGATGTCGAAATCCGC
ACCATCCTGCAGATTTTGGCAAAAGAATCCGGGATGAACATTGTTGCCAGCGACTCCGTCAGCGGCAAAATGACCCTCTC
CCTCAAAGACGTACCTTGGGATCAGGCTTTGGATTTGGTTATGCAGGCGCGCAACCTCGATATGCGCCAGCAAGGGAACA
TCGTCAACATCGCGCCCCGCGACGAGCTGCTTGCCAAAGACAAAGCCTTCTTACAAGCGGAAAAAGACATTGCCGATCTG
GGCGCGCTGTATTCCCAAAACTTCCAATTGAAATACAAAAATGTGGAAGAATTCCGCAGCATCCTGCGTTTGGACAATGC
CGACACGACCGGAAACCGCAACACGCTTGTCAGCGGCAGGGGCAGCGTGCTGATCGATCCCGCCACCAACACCCTGATTG
TTACCGATACCCGCAGCGTCATCGAAAAATTCCGCAAACTGATTGACGAATTGGACGTACCCGCGCAACAAGTGATGATT
GAGGCGCGTATCGTCGAAGCGGCAGACGGCTTCTCGCGCGATTTGGGCGTTAAGTTCGGCGCGACAGGCAGGAAAAAACT
GAAAAATGAGACGAGCGCATTCGGCTGGGGCGTGAACTCCGGCTTCGGGGGCGGCGATAAATGGGAGGCCCAAACCAAAA
TCAACCTGCCGGTTGCCGCTGCCGCAAACAGCATTTCGCTGGTGCGCGCGATTTCCTCCGGCGCGTTGAATTTGGAATTG
TCCGCATCCGAGTCGCTTTCAAAAACCAAAACGCTTGCCAATCCGCGCGTGCTGACCCAAAACCGCAAAGAGGCCAAAAT
CGAATCCGGTTACGAAATTCCTTTTACCGTAACTACAGCCTCGGGCGGCGGCAACTCTACCAACACGGAACTCAAAAAAG
CCGTCTTGGGGCTGACCGTTACGCCGAACATCACGCCCGACGGACAAATCATCATGACCGTCAAAATCAACAAAGACTCG
CCTGCACAATGTGCTTCAGGCAACAACACAATCCTATGTATTTCGACCAAAAGCCTGAATACGCAGGCTATGGTTGAAAA
CGGCGGCACTTTGATTGTCGGCGGTATTTATGAAGAAAACAACGGCAATACGCTGACCAAAGTCCCCCTGTTGGGCGACA
TCCCCGTTATCGGCAACCTCTTTAAAACACGCGGGAAAAAAACCGACCGCCGCGAACTGCTGATTTTCATTACCCCGAGG
ATTATAGATACGGCCGGCAACAGCCTGCGCTATTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(43-116)

Bacterial type II/III secretion system short domain (Secretin_N)

(406-477)

Secretin and TonB N terminus short domain (STN)

(333-378)

Bacterial type II and III secretion system protein (Secretin)

(563-723)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

98.906

100

0.989
  pilQ Neisseria meningitidis MC58

90.897

100

0.956
  pilQ Neisseria meningitidis M1080

91.59

100

0.953