Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   HCU65_RS13245 Genome accession   NZ_CP050705
Coordinates   2674547..2675170 (-) Length   207 a.a.
NCBI ID   WP_219946375.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain PENSV20     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2669547..2680170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCU65_RS13215 (HCU65_13245) rpsT 2669841..2670107 (+) 267 WP_003325396.1 30S ribosomal protein S20 -
  HCU65_RS13220 (HCU65_13250) holA 2670122..2671165 (-) 1044 WP_003325395.1 DNA polymerase III subunit delta -
  HCU65_RS13225 (HCU65_13255) - 2671205..2671354 (-) 150 WP_010789254.1 hypothetical protein -
  HCU65_RS13230 (HCU65_13260) - 2671395..2671529 (+) 135 WP_003229983.1 YqzM family protein -
  HCU65_RS13235 (HCU65_13265) comEC 2671570..2673903 (-) 2334 WP_219946374.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  HCU65_RS13240 (HCU65_13270) - 2673910..2674479 (-) 570 WP_003325392.1 ComE operon protein 2 -
  HCU65_RS13245 (HCU65_13275) comEA 2674547..2675170 (-) 624 WP_219946375.1 helix-hairpin-helix domain-containing protein Machinery gene
  HCU65_RS13250 (HCU65_13280) comER 2675254..2676075 (+) 822 WP_219946376.1 late competence protein ComER -
  HCU65_RS13255 (HCU65_13285) - 2676145..2676885 (-) 741 WP_003325388.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  HCU65_RS13260 (HCU65_13290) rsfS 2676882..2677238 (-) 357 WP_003325387.1 ribosome silencing factor -
  HCU65_RS13265 (HCU65_13295) yqeK 2677255..2677815 (-) 561 WP_010789259.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  HCU65_RS13270 (HCU65_13300) - 2677805..2678374 (-) 570 WP_219946377.1 nicotinate-nucleotide adenylyltransferase -
  HCU65_RS13275 (HCU65_13305) yhbY 2678386..2678676 (-) 291 WP_003325384.1 ribosome assembly RNA-binding protein YhbY -
  HCU65_RS13280 (HCU65_13310) aroE 2678670..2679512 (-) 843 WP_061669959.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22003.80 Da        Isoelectric Point: 4.7220

>NTDB_id=434136 HCU65_RS13245 WP_219946375.1 2674547..2675170(-) (comEA) [Bacillus atrophaeus strain PENSV20]
MNWFYQHKKIILTAGAAVIFIVILLNFPGAKKQEPVQQITASPETAGTRVQQQESKGDADGEKIIIDIKGAVKHPGVYEL
KTGDRVSQAIEKAGGINSEADEKQVNLAELLQDGTVVYIPSEGEEPTQSAAANASVQNGAGKEALVNINTASLEELQAIS
GVGPSKAEAIIAYREENGKFQAVEDITNVSGIGEKSFEKIKSAITVK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=434136 HCU65_RS13245 WP_219946375.1 2674547..2675170(-) (comEA) [Bacillus atrophaeus strain PENSV20]
ATGAATTGGTTTTACCAGCACAAAAAAATAATCTTAACGGCAGGTGCTGCTGTCATCTTCATCGTCATCCTGCTTAATTT
TCCCGGCGCAAAGAAGCAGGAGCCTGTTCAGCAAATCACAGCGTCGCCTGAAACGGCGGGCACACGAGTTCAGCAGCAAG
AATCGAAAGGTGATGCTGACGGAGAAAAAATTATCATTGATATTAAAGGCGCTGTCAAACATCCGGGTGTTTACGAATTG
AAAACGGGAGACAGAGTTTCACAGGCTATCGAAAAAGCGGGAGGCATTAATTCGGAGGCTGATGAAAAACAAGTAAATTT
AGCTGAACTCCTGCAGGACGGCACTGTCGTGTACATTCCGAGTGAGGGGGAAGAACCAACACAAAGCGCTGCAGCAAATG
CTTCTGTGCAAAACGGAGCAGGAAAAGAGGCTTTGGTTAATATCAATACAGCGTCATTAGAGGAACTGCAGGCTATTTCC
GGGGTCGGTCCGTCAAAAGCAGAAGCTATAATAGCTTATCGTGAAGAAAACGGAAAATTTCAAGCCGTTGAAGACATTAC
AAATGTCTCGGGAATCGGCGAAAAATCATTTGAGAAAATAAAATCAGCCATCACAGTTAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(66-120)

Helix-hairpin-helix motif (HHH_3)

(143-205)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

65.7

100

0.657
  comEA Staphylococcus aureus MW2

36.364

100

0.367