Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   HCU65_RS13235 Genome accession   NZ_CP050705
Coordinates   2671570..2673903 (-) Length   777 a.a.
NCBI ID   WP_219946374.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain PENSV20     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2666570..2678903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCU65_RS13200 (HCU65_13230) - 2666901..2667239 (-) 339 WP_004429433.1 YqxA family protein -
  HCU65_RS13205 (HCU65_13235) spoIIP 2667258..2668466 (-) 1209 WP_219946372.1 stage II sporulation protein P -
  HCU65_RS13210 (HCU65_13240) gpr 2668533..2669636 (-) 1104 WP_219946373.1 GPR endopeptidase -
  HCU65_RS13215 (HCU65_13245) rpsT 2669841..2670107 (+) 267 WP_003325396.1 30S ribosomal protein S20 -
  HCU65_RS13220 (HCU65_13250) holA 2670122..2671165 (-) 1044 WP_003325395.1 DNA polymerase III subunit delta -
  HCU65_RS13225 (HCU65_13255) - 2671205..2671354 (-) 150 WP_010789254.1 hypothetical protein -
  HCU65_RS13230 (HCU65_13260) - 2671395..2671529 (+) 135 WP_003229983.1 YqzM family protein -
  HCU65_RS13235 (HCU65_13265) comEC 2671570..2673903 (-) 2334 WP_219946374.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  HCU65_RS13240 (HCU65_13270) - 2673910..2674479 (-) 570 WP_003325392.1 ComE operon protein 2 -
  HCU65_RS13245 (HCU65_13275) comEA 2674547..2675170 (-) 624 WP_219946375.1 helix-hairpin-helix domain-containing protein Machinery gene
  HCU65_RS13250 (HCU65_13280) comER 2675254..2676075 (+) 822 WP_219946376.1 late competence protein ComER -
  HCU65_RS13255 (HCU65_13285) - 2676145..2676885 (-) 741 WP_003325388.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  HCU65_RS13260 (HCU65_13290) rsfS 2676882..2677238 (-) 357 WP_003325387.1 ribosome silencing factor -
  HCU65_RS13265 (HCU65_13295) yqeK 2677255..2677815 (-) 561 WP_010789259.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  HCU65_RS13270 (HCU65_13300) - 2677805..2678374 (-) 570 WP_219946377.1 nicotinate-nucleotide adenylyltransferase -
  HCU65_RS13275 (HCU65_13305) yhbY 2678386..2678676 (-) 291 WP_003325384.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 777 a.a.        Molecular weight: 86977.83 Da        Isoelectric Point: 8.6349

>NTDB_id=434134 HCU65_RS13235 WP_219946374.1 2671570..2673903(-) (comEC) [Bacillus atrophaeus strain PENSV20]
MKNTRLLLPLAAASATAGIAAASFFSALLIFVLSLFFVYIKTKQLSLVFVSLSAFTIYFFLTSVIDSHNVSSYDIGTYKL
RAVIQEIPKIDGDRMSMVVRTPDGEKWASSYKMESFAEKEKLSQIEPGTACTFTGSLKKPKNATVPGTFDYKQYLYSQHI
HWNYSITAIQNCSTADGIQYRLLSVRKSLISFVNDHVPESSAGIVQALILGDRFYLMDDVLEAYQKLGVVHLLAISGLHV
GIVTAGIFYVLIRIGVTREKAAILLLLFLPVYTILTGAAPSVMRAALMSGLYFIGSLWGKRMNPSIVICLSYLILLLFNP
YHLFQIGFQLSFAVSFSLILSSSIFKKLQSGGLVQLAMVSLIAQLGSLPILIYYFQQFSLISIPMNIIMVPFYTMFVMPF
SMIGFLLLSLSEPVGEYLFYWFDYIMCWSDKLIKYAADIEQFTVIAARPNALVLFGLIGSIVVLLTAMEKSPLYQRLLSV
LLFIAMLFLVVIFPRLDPEGEVDMLDIGQGDSMFISAPYQRGHVMIDTGGILSYLTEPWREKRHPFSLSEKVLIPFLTAK
GIKQLDALILTHADQDHIGEAKVLIKRHKVKQLIVPKGFVSEPNDEKVLRTAQQEGICITEVKRGDTFQIKDLQFRVLSP
ETPDTNSKNNSSLVLWMKTGHLSWVLTGDLEKEGEQEIMKAFPDLKADILKVGHHGSNGSTGEEFVRHLKPKAAIISAGE
NNRYHHPHQEVLDILKAHSVRIFRTDQSGTIQYRFKSGFGTFSVYPPYDTTGMTETN

Nucleotide


Download         Length: 2334 bp        

>NTDB_id=434134 HCU65_RS13235 WP_219946374.1 2671570..2673903(-) (comEC) [Bacillus atrophaeus strain PENSV20]
ATGAAGAACACACGCCTTCTTTTGCCTCTGGCGGCAGCATCAGCAACGGCTGGCATAGCTGCTGCCTCTTTTTTCTCCGC
ACTTCTCATATTCGTTTTATCACTCTTCTTTGTTTATATCAAAACAAAACAGCTCTCACTCGTTTTTGTAAGTTTATCGG
CTTTTACGATCTATTTTTTCTTAACCTCTGTTATCGATTCTCATAATGTATCGTCCTATGACATCGGCACCTATAAGCTT
CGGGCGGTGATTCAGGAAATTCCAAAGATTGACGGAGACCGTATGTCCATGGTAGTTCGAACGCCGGATGGGGAAAAGTG
GGCTTCATCGTATAAAATGGAGTCGTTTGCTGAAAAGGAAAAACTTTCACAGATAGAGCCAGGGACGGCTTGTACGTTTA
CCGGTTCTTTAAAAAAACCGAAAAACGCAACGGTTCCCGGCACTTTCGATTATAAACAGTATCTGTACAGCCAGCATATT
CACTGGAATTACTCGATAACAGCAATCCAAAATTGCAGCACGGCAGATGGAATACAGTATAGGCTTTTAAGCGTGAGAAA
GTCTCTTATTTCATTTGTGAATGACCATGTTCCGGAATCCTCAGCAGGCATCGTGCAGGCGCTTATCCTGGGTGACAGAT
TTTATCTTATGGATGATGTGCTGGAAGCGTATCAAAAGCTCGGAGTTGTCCATCTGCTTGCGATTTCAGGGCTTCATGTA
GGTATTGTGACGGCTGGTATATTCTATGTGCTCATTCGAATTGGGGTAACGAGAGAGAAGGCTGCCATTCTGCTGCTGTT
GTTTTTGCCTGTTTATACGATTTTAACCGGAGCGGCTCCGTCTGTGATGCGCGCAGCTCTTATGTCAGGGCTTTATTTTA
TCGGCAGTTTGTGGGGAAAGCGTATGAACCCTTCAATAGTGATCTGTCTTTCTTATTTAATTCTCCTTTTGTTTAATCCG
TATCATCTTTTTCAAATAGGGTTTCAGCTCTCATTTGCCGTAAGTTTCTCTCTAATACTTTCTTCCTCTATTTTTAAAAA
ACTTCAAAGCGGGGGACTCGTGCAGCTCGCCATGGTGTCACTCATCGCTCAACTTGGATCGCTTCCCATCCTTATCTATT
ATTTTCAGCAGTTTTCTCTTATCAGTATACCGATGAATATCATTATGGTGCCTTTTTATACGATGTTTGTCATGCCGTTT
TCTATGATAGGTTTTCTTTTGTTAAGTTTGTCTGAGCCGGTCGGTGAGTACCTGTTTTATTGGTTTGATTACATCATGTG
CTGGAGTGACAAGCTGATAAAGTATGCTGCAGACATTGAGCAATTTACAGTGATTGCAGCCCGTCCGAATGCGCTTGTCT
TGTTTGGGCTGATTGGATCTATCGTTGTTTTGCTGACAGCCATGGAAAAATCCCCCCTTTACCAAAGACTTCTGTCTGTG
CTGCTCTTTATCGCAATGCTTTTTCTGGTGGTTATTTTTCCTCGCCTGGACCCCGAGGGAGAAGTGGATATGCTTGATAT
CGGTCAGGGAGACAGCATGTTTATCAGCGCTCCGTATCAGCGCGGCCATGTGATGATAGACACTGGAGGAATCCTTTCAT
ACTTAACAGAGCCATGGAGGGAAAAACGGCATCCTTTTTCGCTTAGCGAAAAGGTGCTCATTCCTTTCTTAACAGCAAAA
GGCATTAAACAGCTTGATGCCTTAATTTTGACTCATGCGGACCAAGACCATATAGGGGAAGCAAAGGTTCTGATCAAGCG
GCATAAAGTCAAACAGCTCATTGTGCCGAAAGGATTTGTTTCTGAACCGAATGATGAAAAAGTGCTGCGGACCGCACAGC
AAGAGGGAATTTGCATTACAGAGGTTAAACGAGGCGACACCTTTCAGATAAAGGATTTACAGTTTCGCGTACTGTCGCCT
GAGACTCCCGACACGAACAGCAAAAATAACTCTTCTCTCGTGCTATGGATGAAAACAGGTCATCTCAGCTGGGTGCTGAC
AGGCGATCTCGAAAAGGAAGGGGAACAGGAGATCATGAAAGCTTTTCCGGATCTAAAAGCGGATATTTTAAAGGTGGGGC
ATCATGGCAGTAATGGATCTACCGGAGAAGAGTTTGTCCGCCACCTGAAACCAAAGGCGGCAATTATTTCTGCCGGTGAG
AATAATCGGTATCACCATCCCCATCAGGAAGTCCTTGATATATTGAAAGCTCATTCTGTCCGCATTTTCCGCACTGATCA
AAGCGGGACGATCCAATACAGATTTAAAAGCGGGTTTGGAACCTTTTCTGTCTATCCCCCATATGATACAACAGGTATGA
CGGAGACGAACTAA

Domains

Predicted by InterproScan.

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)

Competence protein (Competence)

(208-467)

Domain of unknown function (DUF4131) (DUF4131)

(25-169)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

65.38

100

0.654