NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	HCC49_RS11885	Genome accession	NZ_CP050462
Coordinates	2446862..2447476 (-)	Length	204 a.a.
NCBI ID	WP_013352942.1	Uniprot ID	A0A411A874
Organism	Bacillus velezensis strain Htq6
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2441862..2452476

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HCC49_RS11855 (HCC49_11855)	rpsT	2442162..2442428 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
HCC49_RS11860 (HCC49_11860)	holA	2442445..2443486 (-)	1042	Protein_2309	DNA polymerase III subunit delta	-
HCC49_RS11865 (HCC49_11865)	-	2443526..2443680 (-)	155	Protein_2310	hypothetical protein	-
HCC49_RS11870 (HCC49_11870)	-	2443721..2443855 (+)	135	WP_003152870.1	YqzM family protein	-
HCC49_RS11875 (HCC49_11875)	comEC	2443874..2446225 (-)	2352	WP_167860288.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HCC49_RS11880 (HCC49_11880)	-	2446226..2446795 (-)	570	WP_003152868.1	ComE operon protein 2	-
HCC49_RS11885 (HCC49_11885)	comEA	2446862..2447476 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
HCC49_RS11890 (HCC49_11890)	comER	2447534..2448355 (+)	822	WP_014305449.1	late competence protein ComER	-
HCC49_RS11895 (HCC49_11895)	-	2448424..2449161 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
HCC49_RS11900 (HCC49_11900)	rsfS	2449158..2449514 (-)	357	WP_003152864.1	ribosome silencing factor	-
HCC49_RS11905 (HCC49_11905)	yqeK	2449532..2450092 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
HCC49_RS11910 (HCC49_11910)	-	2450082..2450651 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
HCC49_RS11915 (HCC49_11915)	yhbY	2450662..2450952 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
HCC49_RS11920 (HCC49_11920)	aroE	2450946..2451788 (-)	843	WP_013352947.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22190.27 Da Isoelectric Point: 5.4841

                                >NTDB_id=432966 HCC49_RS11885 WP_013352942.1 2446862..2447476(-) (comEA) [Bacillus velezensis strain Htq6]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=432966 HCC49_RS11885 WP_013352942.1 2446862..2447476(-) (comEA) [Bacillus velezensis strain Htq6]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAATCCGTGCTCGGCCAAACAGCCTCGGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0432966