Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BVELS4_RS12200 Genome accession   NZ_CP050424
Coordinates   2534261..2535331 (-) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus velezensis strain S4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2529261..2540331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVELS4_RS12150 (BVELS4_02438) - 2529321..2529905 (-) 585 WP_025284996.1 signal peptidase I -
  BVELS4_RS12155 (BVELS4_02439) tapA 2529877..2530548 (-) 672 WP_015240206.1 amyloid fiber anchoring/assembly protein TapA -
  BVELS4_RS12160 (BVELS4_02440) - 2530807..2531136 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  BVELS4_RS12165 (BVELS4_02441) - 2531176..2531355 (-) 180 WP_003153093.1 YqzE family protein -
  BVELS4_RS12170 (BVELS4_02442) comGG 2531412..2531789 (-) 378 WP_167340753.1 competence type IV pilus minor pilin ComGG Machinery gene
  BVELS4_RS12175 (BVELS4_02443) comGF 2531790..2532290 (-) 501 WP_257720329.1 competence type IV pilus minor pilin ComGF -
  BVELS4_RS12180 (BVELS4_02444) comGE 2532199..2532513 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  BVELS4_RS12185 (BVELS4_02445) comGD 2532497..2532934 (-) 438 WP_043020787.1 competence type IV pilus minor pilin ComGD Machinery gene
  BVELS4_RS12190 (BVELS4_02446) comGC 2532924..2533190 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  BVELS4_RS12195 (BVELS4_02447) comGB 2533237..2534274 (-) 1038 WP_015240211.1 competence type IV pilus assembly protein ComGB Machinery gene
  BVELS4_RS12200 (BVELS4_02448) comGA 2534261..2535331 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  BVELS4_RS12205 (BVELS4_02449) - 2535523..2536473 (-) 951 WP_032870601.1 magnesium transporter CorA family protein -
  BVELS4_RS12210 (BVELS4_02450) - 2536619..2537922 (+) 1304 Protein_2364 hemolysin family protein -
  BVELS4_RS12215 (BVELS4_02451) - 2537960..2538790 (-) 831 WP_015240214.1 STAS domain-containing protein -
  BVELS4_RS12225 (BVELS4_02453) - 2538997..2539377 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BVELS4_RS12230 (BVELS4_02454) - 2539614..2539859 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=432570 BVELS4_RS12200 WP_012117985.1 2534261..2535331(-) (comGA) [Bacillus velezensis strain S4]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=432570 BVELS4_RS12200 WP_012117985.1 2534261..2535331(-) (comGA) [Bacillus velezensis strain S4]
GTGAAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374