Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DSUI_RS03425 Genome accession   NC_016616
Coordinates   698768..700246 (-) Length   492 a.a.
NCBI ID   WP_014235814.1    Uniprot ID   G8QH21
Organism   Azospira oryzae PS     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 693768..705246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSUI_RS03395 (Dsui_0698) - 694903..695718 (-) 816 WP_014235808.1 hypothetical protein -
  DSUI_RS03400 (Dsui_0699) - 695735..696406 (-) 672 WP_014235809.1 hypothetical protein -
  DSUI_RS03405 (Dsui_0700) - 696528..696842 (-) 315 WP_014235810.1 PsiF family protein -
  DSUI_RS03410 (Dsui_0701) hprK 696888..697823 (-) 936 WP_014235811.1 HPr(Ser) kinase/phosphatase -
  DSUI_RS03415 (Dsui_0702) ptsN 697792..698277 (-) 486 WP_014235812.1 PTS IIA-like nitrogen regulatory protein PtsN -
  DSUI_RS03420 (Dsui_0703) hpf 698433..698753 (-) 321 WP_014235813.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DSUI_RS03425 (Dsui_0704) rpoN/rpoN1 698768..700246 (-) 1479 WP_014235814.1 RNA polymerase factor sigma-54 Machinery gene
  DSUI_RS03430 (Dsui_0705) lptB 700313..701059 (-) 747 WP_014235815.1 LPS export ABC transporter ATP-binding protein -
  DSUI_RS03435 (Dsui_0706) recX 701125..701580 (-) 456 WP_014235816.1 recombination regulator RecX -
  DSUI_RS03440 (Dsui_0707) recA 701577..702599 (-) 1023 WP_014235817.1 recombinase RecA Machinery gene
  DSUI_RS03445 (Dsui_0708) - 702724..704817 (+) 2094 WP_014235818.1 PAS domain S-box protein -

Sequence

Protein


Download         Length: 492 a.a.        Molecular weight: 55162.93 Da        Isoelectric Point: 4.6688

>NTDB_id=43126 DSUI_RS03425 WP_014235814.1 698768..700246(-) (rpoN/rpoN1) [Azospira oryzae PS]
MKPTLQLKLSQHLALTPQLQQSIRLLQLSTLELEQELEKYLLENPLLEREDENYEPSYSGSEGEYAGSTAEAAAEGPREG
DSGESGEGADSHGPEEVRDDSRDSLGEGEDWFAEGSYPSASRDEDEDSDFQEVQAANTSLRDHLNWQLGLMQLSERDRQL
VSFLIEALDEDGYLTQPLEELQEALPEELEIDLDDLQIALRHLQHLDPTGVGARTPGECLALQLEALPADEVRDQALKLV
RHHLELLAGRDFTKLKKLLGCDDERLRAVTNLVKSLNPRPGAQYAPLDARYITPDVVVKKVRNQWTASINPDAYPRLRIN
RLYAEILSKQRGSSLSGQLQEARWLIKNVQQRFDTILRVSQAILDRQRQFFEHGEVAMRPLVLREIAETLGLHESTVSRV
TTQKYMATPRGIFELKYFFGSHVATDTGGACSATAIRALIKQLVAAEDPRKPLSDSQISEILGQQGIVVARRTIAKYREA
LNISPANLRKSI

Nucleotide


Download         Length: 1479 bp        

>NTDB_id=43126 DSUI_RS03425 WP_014235814.1 698768..700246(-) (rpoN/rpoN1) [Azospira oryzae PS]
ATGAAACCAACCCTCCAACTCAAACTCTCGCAGCACCTGGCCCTGACGCCACAGCTGCAGCAGTCCATCCGGCTGCTGCA
ACTGTCGACGCTCGAGCTGGAGCAAGAGTTGGAGAAGTATCTGCTGGAAAACCCCCTGCTGGAGCGGGAGGACGAGAACT
ACGAGCCCTCCTACAGCGGGAGCGAAGGCGAATACGCCGGCAGTACGGCCGAGGCCGCTGCCGAAGGCCCGCGGGAAGGC
GATAGCGGCGAATCCGGTGAAGGCGCCGACAGTCATGGCCCCGAAGAAGTCCGGGACGACAGCCGGGACAGCCTGGGCGA
AGGCGAGGACTGGTTTGCCGAAGGCAGCTACCCTTCCGCCTCCCGCGACGAAGACGAGGATTCCGACTTCCAGGAAGTGC
AGGCCGCCAACACCTCCCTGCGCGACCACCTCAACTGGCAGCTGGGCCTGATGCAGCTCTCCGAGCGGGACCGGCAGCTG
GTCAGCTTCCTCATCGAGGCGCTGGACGAGGACGGCTACCTGACCCAGCCCCTGGAGGAGCTGCAGGAAGCCCTGCCGGA
AGAGCTGGAAATCGACCTGGACGACCTGCAGATCGCCCTGCGCCACCTGCAGCACCTGGACCCGACCGGTGTCGGCGCCC
GTACTCCGGGCGAATGCCTGGCCCTGCAGCTGGAAGCCCTGCCGGCCGACGAGGTGCGGGACCAGGCCTTGAAGCTGGTG
CGCCATCATCTGGAACTGCTGGCCGGCCGGGACTTCACCAAGCTGAAGAAGCTGCTGGGCTGCGACGACGAGCGCCTGCG
CGCCGTCACCAACCTGGTGAAGAGCCTCAACCCCCGGCCCGGCGCCCAGTACGCGCCCCTGGATGCCCGCTACATCACCC
CGGACGTGGTGGTGAAGAAGGTGCGCAACCAGTGGACCGCCAGCATCAATCCGGACGCCTACCCGCGCCTACGCATCAAC
CGGCTCTACGCCGAGATCCTCTCCAAGCAGCGCGGCTCCAGCCTCTCCGGCCAGCTGCAGGAGGCCCGCTGGCTGATCAA
GAACGTGCAGCAGCGTTTCGACACCATCCTGCGCGTGTCCCAGGCCATCCTGGACCGCCAGCGCCAGTTCTTCGAACACG
GCGAGGTGGCCATGCGTCCCCTGGTGCTGCGGGAAATCGCCGAAACCCTGGGCCTGCACGAATCCACCGTCTCCCGGGTG
ACCACCCAGAAGTACATGGCCACCCCCCGGGGCATTTTCGAACTCAAGTATTTCTTCGGCAGCCATGTGGCCACGGATAC
GGGGGGCGCCTGTTCGGCCACCGCCATCCGGGCCCTGATCAAGCAGCTGGTGGCGGCCGAAGACCCCAGAAAACCCCTTT
CGGACAGTCAAATATCCGAAATTCTTGGCCAACAAGGCATTGTTGTGGCGCGTCGAACCATTGCAAAATACAGAGAAGCG
CTCAACATTTCGCCGGCCAACCTACGCAAGTCCATCTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-490)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G8QH21

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

56.805

100

0.585

Multiple sequence alignment