Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VEJY3_RS14260 Genome accession   NC_016613
Coordinates   3147754..3148770 (-) Length   338 a.a.
NCBI ID   WP_014233152.1    Uniprot ID   -
Organism   Vibrio sp. EJY3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3142754..3153770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VEJY3_RS14235 (VEJY3_14045) aroK 3143626..3144144 (-) 519 WP_014233147.1 shikimate kinase AroK -
  VEJY3_RS14240 (VEJY3_14050) pilQ 3144333..3146036 (-) 1704 WP_014233148.1 type IV pilus secretin PilQ Machinery gene
  VEJY3_RS14245 (VEJY3_14055) pilP 3146104..3146619 (-) 516 WP_014233149.1 pilus assembly protein PilP Machinery gene
  VEJY3_RS14250 (VEJY3_14060) pilO 3146609..3147202 (-) 594 WP_014233150.1 type 4a pilus biogenesis protein PilO Machinery gene
  VEJY3_RS14255 (VEJY3_14065) pilN 3147195..3147770 (-) 576 WP_014233151.1 PilN domain-containing protein Machinery gene
  VEJY3_RS14260 (VEJY3_14070) pilM 3147754..3148770 (-) 1017 WP_014233152.1 type IV pilus assembly protein PilM Machinery gene
  VEJY3_RS14265 (VEJY3_14075) - 3148914..3151481 (+) 2568 WP_014233153.1 penicillin-binding protein 1A -
  VEJY3_RS14270 (VEJY3_14080) oxyR 3151546..3152454 (-) 909 WP_014233154.1 DNA-binding transcriptional regulator OxyR -
  VEJY3_RS14275 (VEJY3_14085) - 3152613..3153341 (+) 729 WP_014233155.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37622.83 Da        Isoelectric Point: 7.2508

>NTDB_id=43110 VEJY3_RS14260 WP_014233152.1 3147754..3148770(-) (pilM) [Vibrio sp. EJY3]
MDKRIVTGIDIGHNSLKAVVLKPNGEQFALLGYKEILLKDGIVAENNTINHQEIVNTLKQVKKDLPFGASRVAISVPDNA
VISKKLHIEQDLEENEIEFAVVQAFSHQSPFPVDELSLDFVRLNHEGGLRGPVSYQVFATRKDVVESRVEAIRKSGFKPV
LVEVHSQSLGQIWKLATERFPDKSQYGLLDVGSVSSSFTMFTEQNELFHKEFACGTGSIHCSVQESPLSGEEQAKTSQFN
RQMVDRVKRQIQLYTSINGSQNIKGLWLSGEGSTTPMLAEELSHQLAVECELLNPLGLFQMNVAKRKRRAAEWQHFSTAA
GLAVRGIQWLGGRRALSN

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=43110 VEJY3_RS14260 WP_014233152.1 3147754..3148770(-) (pilM) [Vibrio sp. EJY3]
ATGGATAAGCGAATCGTTACAGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTCGTACTTAAACCCAATGGCGAGCA
ATTCGCCTTGTTGGGTTACAAAGAAATATTACTTAAGGATGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAATACCCTTAAACAGGTGAAAAAAGACCTGCCATTTGGTGCAAGCAGAGTGGCAATTTCAGTCCCTGATAATGCA
GTTATCAGCAAAAAACTGCACATCGAACAAGACCTTGAAGAGAACGAAATAGAGTTCGCGGTCGTGCAGGCATTTTCTCA
TCAATCTCCTTTCCCTGTAGATGAGTTGAGTCTGGACTTTGTCCGTCTGAATCATGAAGGAGGCCTGCGAGGGCCAGTTA
GCTACCAAGTTTTTGCGACACGTAAAGATGTCGTGGAGAGTCGGGTTGAAGCAATACGTAAATCCGGTTTTAAACCTGTT
CTCGTCGAGGTCCATTCACAAAGCCTCGGGCAGATATGGAAACTGGCCACAGAACGTTTTCCAGATAAAAGTCAGTACGG
TTTGCTTGATGTTGGTTCTGTGTCGAGCTCTTTCACGATGTTCACCGAGCAAAACGAGCTATTTCATAAAGAGTTTGCCT
GTGGAACAGGTTCCATTCATTGCTCTGTGCAGGAAAGCCCTCTGTCCGGAGAAGAACAAGCCAAAACGAGTCAATTCAAT
CGTCAGATGGTCGATCGTGTCAAACGCCAGATACAGCTTTATACGTCCATTAACGGATCACAAAACATCAAAGGTCTTTG
GTTATCCGGTGAAGGGTCAACAACGCCAATGCTGGCTGAGGAGCTATCTCATCAATTGGCGGTCGAGTGCGAGTTACTGA
ACCCGTTAGGCTTATTTCAAATGAACGTCGCTAAACGTAAGCGTCGAGCGGCAGAGTGGCAGCACTTTTCAACCGCCGCT
GGCTTGGCAGTACGAGGTATCCAATGGTTAGGAGGTCGCCGTGCTTTATCGAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(239-326)

Type IV pilus assembly protein PilM; (PilM_2)

(8-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

74.852

100

0.749
  pilM Vibrio cholerae strain A1552

48.225

100

0.482

Multiple sequence alignment