Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   G4G26_RS12760 Genome accession   NZ_CP049924
Coordinates   2503594..2504211 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain So1b     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2498594..2509211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4G26_RS12730 (G4G26_12765) rpsT 2498903..2499169 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  G4G26_RS12735 (G4G26_12770) holA 2499184..2500227 (-) 1044 WP_015714286.1 DNA polymerase III subunit delta -
  G4G26_RS12740 (G4G26_12775) - 2500267..2500416 (-) 150 WP_003229985.1 hypothetical protein -
  G4G26_RS12745 (G4G26_12780) yqzM 2500457..2500591 (+) 135 WP_003229983.1 YqzM family protein -
  G4G26_RS12750 (G4G26_12785) comEC 2500631..2502961 (-) 2331 WP_074794436.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  G4G26_RS12755 (G4G26_12790) comEB 2502958..2503527 (-) 570 WP_015714288.1 ComE operon protein 2 -
  G4G26_RS12760 (G4G26_12795) comEA 2503594..2504211 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  G4G26_RS12765 (G4G26_12800) comER 2504295..2505116 (+) 822 WP_014480313.1 late competence protein ComER -
  G4G26_RS12770 (G4G26_12805) yqeM 2505182..2505925 (-) 744 WP_069322765.1 class I SAM-dependent methyltransferase -
  G4G26_RS12775 (G4G26_12810) rsfS 2505922..2506278 (-) 357 WP_041333759.1 ribosome silencing factor -
  G4G26_RS12780 (G4G26_12815) yqeK 2506296..2506856 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  G4G26_RS12785 (G4G26_12820) nadD 2506846..2507415 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  G4G26_RS12790 (G4G26_12825) yhbY 2507427..2507717 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  G4G26_RS12795 (G4G26_12830) aroE 2507711..2508553 (-) 843 WP_088467268.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=428745 G4G26_RS12760 WP_004398514.1 2503594..2504211(-) (comEA) [Bacillus subtilis strain So1b]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=428745 G4G26_RS12760 WP_004398514.1 2503594..2504211(-) (comEA) [Bacillus subtilis strain So1b]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTCTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39