Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   G8D99_RS01325 Genome accession   NZ_CP049916
Coordinates   284476..285102 (+) Length   208 a.a.
NCBI ID   WP_166321911.1    Uniprot ID   A0A6G8S1D8
Organism   Acinetobacter lanii strain 185     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 279476..290102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8D99_RS01310 (G8D99_01310) - 279814..280620 (+) 807 WP_166327430.1 putative RNA methyltransferase -
  G8D99_RS01315 (G8D99_01315) ponA 280701..283253 (-) 2553 WP_166321907.1 penicillin-binding protein PBP1a -
  G8D99_RS01320 (G8D99_01320) comM 283418..284476 (+) 1059 WP_166321909.1 pilus assembly protein PilM Machinery gene
  G8D99_RS01325 (G8D99_01325) pilN 284476..285102 (+) 627 WP_166321911.1 PilN domain-containing protein Machinery gene
  G8D99_RS01330 (G8D99_01330) comO 285099..285857 (+) 759 WP_166321913.1 type 4a pilus biogenesis protein PilO Machinery gene
  G8D99_RS01335 (G8D99_01335) pilP 285854..286381 (+) 528 WP_166321915.1 pilus assembly protein PilP Machinery gene
  G8D99_RS01340 (G8D99_01340) pilQ 286401..288590 (+) 2190 WP_166321917.1 type IV pilus secretin PilQ Machinery gene
  G8D99_RS01345 (G8D99_01345) aroK 288627..289175 (+) 549 WP_166321919.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 208 a.a.        Molecular weight: 23239.51 Da        Isoelectric Point: 5.1171

>NTDB_id=428624 G8D99_RS01325 WP_166321911.1 284476..285102(+) (pilN) [Acinetobacter lanii strain 185]
MAKINLLPWRDELREQRKKQFITICIAVALLAIASVLGTWFYLNQKLDDQEQANQLVTSTNQNLDTQLKTLDGLQERRNS
VIERMKLIQGLEGQRPVTVRLVDELVRVMPSTVYVTKFSRVGDKFTIEGNAESPNSVAELLRGLEASEWYRNAFMNSFLA
APEVKDKAPSSVVPRVEESYGTFVVTADIGEIALPAPTDSNNNKGATP

Nucleotide


Download         Length: 627 bp        

>NTDB_id=428624 G8D99_RS01325 WP_166321911.1 284476..285102(+) (pilN) [Acinetobacter lanii strain 185]
ATGGCAAAGATTAATTTACTCCCTTGGCGTGATGAGCTAAGAGAACAACGAAAAAAACAATTTATCACCATTTGTATCGC
TGTTGCGTTATTGGCCATTGCTTCGGTCTTGGGGACTTGGTTTTATTTGAATCAAAAGTTGGATGATCAAGAACAAGCCA
ATCAACTGGTGACCAGTACCAATCAAAATTTGGATACCCAACTCAAAACCCTAGATGGTCTGCAAGAACGCCGTAATAGT
GTGATTGAACGGATGAAACTGATTCAAGGTCTAGAAGGGCAACGCCCAGTGACTGTGCGTTTGGTTGATGAGTTGGTTCG
GGTGATGCCTAGTACGGTTTATGTGACCAAATTTAGCCGTGTTGGGGATAAATTTACCATTGAAGGCAATGCCGAAAGTC
CGAACAGTGTCGCTGAATTACTACGTGGCTTAGAAGCCTCCGAATGGTATCGCAATGCCTTTATGAACTCGTTCTTGGCA
GCGCCAGAAGTCAAAGACAAAGCGCCAAGTTCAGTGGTTCCGCGTGTTGAAGAATCCTATGGCACCTTTGTCGTGACGGC
GGATATTGGGGAAATTGCATTACCGGCGCCAACAGATTCAAATAATAATAAGGGGGCTACGCCATGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-159)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8S1D8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Acinetobacter baumannii D1279779

73.5

96.154

0.707
  comN Acinetobacter baylyi ADP1

70.984

92.788

0.659