Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   IHN46_RS15365 Genome accession   NZ_CP062249
Coordinates   3164386..3165045 (+) Length   219 a.a.
NCBI ID   WP_001221495.1    Uniprot ID   P52076
Organism   Escherichia coli strain AML002_ev01     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3159386..3170045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHN46_RS15340 ygiS 3160669..3162276 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  IHN46_RS15345 mqsA 3162409..3162804 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  IHN46_RS15350 mqsR 3162806..3163102 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  IHN46_RS15355 ygiV 3163307..3163789 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  IHN46_RS15360 ygiW 3163842..3164234 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  IHN46_RS15365 ciaR 3164386..3165045 (+) 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB Regulator
  IHN46_RS15370 qseC 3165042..3166391 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  IHN46_RS15375 ygiZ 3166437..3166769 (-) 333 WP_000917684.1 DUF2645 family protein -
  IHN46_RS15380 mdaB 3167088..3167669 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  IHN46_RS15385 ygiN 3167700..3168014 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  IHN46_RS15390 parE 3168062..3169954 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24677.59 Da        Isoelectric Point: 6.9850

>NTDB_id=425200 IHN46_RS15365 WP_001221495.1 3164386..3165045(+) (ciaR) [Escherichia coli strain AML002_ev01]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=425200 IHN46_RS15365 WP_001221495.1 3164386..3165045(+) (ciaR) [Escherichia coli strain AML002_ev01]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P52076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361