Detailed information NTG0424404

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ATY02_RS31290
• Genome accession: NZ_CP049045
• Coordinates: 6734737..6735609 (+)
• Length: 290 a.a.
• NCBI ID: WP_068575527.1
• Uniprot ID: A0A6G7A7M5
• Organism: Pseudomonas sp. BIOMIG1BAC
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6729737..6740609

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ATY02_RS31275 (ATY02_31155)	-	6732253..6732609 (-)	357	WP_068575524.1	BON domain-containing protein	-
ATY02_RS31280 (ATY02_31160)	-	6732826..6733242 (-)	417	WP_068575525.1	pilin	-
ATY02_RS31285 (ATY02_31165)	pilC	6733517..6734734 (+)	1218	WP_068575526.1	type II secretion system F family protein	Machinery gene
ATY02_RS31290 (ATY02_31170)	pilD	6734737..6735609 (+)	873	WP_068575527.1	prepilin peptidase	Machinery gene
ATY02_RS31295 (ATY02_31175)	coaE	6735606..6736229 (+)	624	WP_068575528.1	dephospho-CoA kinase	-
ATY02_RS31300 (ATY02_31180)	yacG	6736226..6736429 (+)	204	WP_068575529.1	DNA gyrase inhibitor YacG	-
ATY02_RS31305 (ATY02_31185)	-	6736447..6736662 (-)	216	WP_022641062.1	hypothetical protein	-
ATY02_RS31310 (ATY02_31190)	-	6736726..6737415 (-)	690	WP_068575530.1	energy-coupling factor ABC transporter permease	-
ATY02_RS31315 (ATY02_31195)	-	6737412..6737879 (-)	468	WP_068575531.1	hypothetical protein	-
ATY02_RS31320 (ATY02_31200)	-	6738032..6738661 (+)	630	WP_068575532.1	DUF1780 domain-containing protein	-
ATY02_RS31325 (ATY02_31205)	-	6738849..6739022 (+)	174	WP_068575533.1	DUF3094 family protein	-
ATY02_RS31330 (ATY02_31210)	-	6739083..6740381 (+)	1299	WP_068575534.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32119.31 Da
• Isoelectric Point: 6.8225

>NTDB_id=424404 ATY02_RS31290 WP_068575527.1 6734737..6735609(+) (pilD) [Pseudomonas sp. BIOMIG1BAC]
MSLPEVLSLYPLVFVMTGLLLGLIVGSFLNVLVWRLPQMLERDWRAQAHEILGLPAEPEIAPYNLLLPHSHCPSCGTPIR
PWQNVPLISYLWLRGRCAHCQTSISLRYPLTELACGLLSAFVAWHFGFGWPAGLMLLLSWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIVNSFGIFTDLENALWGAVIGYLFLWSVFWLFKLLTGKDGLGHGDFKLLALLGAWGGWQILPLTLLLASLTG
ALLGTLILRLRQAKGSTPIPFGPFLAIAGWIALLWGGQITDFYLQFVGFQ

Nucleotide

• Length: 873 bp

>NTDB_id=424404 ATY02_RS31290 WP_068575527.1 6734737..6735609(+) (pilD) [Pseudomonas sp. BIOMIG1BAC]
ATGTCCTTGCCTGAAGTCCTTAGCCTTTATCCCCTGGTATTCGTAATGACCGGCCTACTGCTGGGGCTGATCGTCGGCAG
TTTTCTCAATGTACTGGTCTGGCGTCTGCCACAGATGCTCGAGCGTGACTGGCGGGCCCAAGCCCATGAAATCCTGGGGC
TACCTGCGGAGCCTGAAATCGCTCCCTATAACCTGCTGTTGCCCCATTCCCATTGCCCCAGCTGCGGAACACCTATCCGT
CCCTGGCAGAATGTTCCGCTGATCAGCTATCTGTGGCTGCGTGGCCGCTGCGCCCATTGCCAAACCAGCATCAGCCTGCG
CTATCCCCTGACCGAACTGGCTTGCGGCCTGCTCTCGGCGTTCGTAGCCTGGCATTTCGGTTTTGGCTGGCCAGCCGGCT
TGATGCTGCTTCTGAGCTGGGGCTTGCTGGCCATGAGCCTGATCGACGCCGACCATCAGTTGCTACCGGATGTTCTGGTA
CTGCCGTTGCTGTGGCTGGGACTGATCGTCAACAGCTTCGGTATCTTCACAGACCTTGAGAATGCCCTGTGGGGCGCGGT
GATCGGCTACTTGTTCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGCTGACCGGCAAGGATGGCCTGGGCCATGGTGATT
TCAAGCTGCTGGCGCTGCTGGGTGCCTGGGGCGGTTGGCAGATCCTGCCGCTGACCCTGCTTCTGGCCTCCCTGACCGGG
GCGCTGCTGGGCACCCTCATATTGCGCTTGCGCCAGGCCAAAGGCTCGACGCCTATCCCCTTTGGCCCATTTCTGGCGAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGACTTCTATTTGCAGTTTGTCGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A6G7A7M5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.709	97.241	0.493
pilD	Vibrio campbellii strain DS40M4	48.582	97.241	0.472
pilD	Acinetobacter nosocomialis M2	50.936	92.069	0.469
pilD	Acinetobacter baumannii D1279779	50.562	92.069	0.466
pilD	Neisseria gonorrhoeae MS11	49.802	87.241	0.434