Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   G4V50_RS08715 Genome accession   NZ_CP048899
Coordinates   1686017..1686877 (+) Length   286 a.a.
NCBI ID   WP_003689814.1    Uniprot ID   A0AA44U9R8
Organism   Neisseria gonorrhoeae strain SRRSH214     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1681017..1691877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4V50_RS08700 dapD 1681527..1682348 (-) 822 WP_003689804.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  G4V50_RS08705 pgi 1682558..1684201 (-) 1644 WP_003689806.1 glucose-6-phosphate isomerase -
  G4V50_RS08710 pilG 1684711..1685943 (+) 1233 WP_003689811.1 type 4 pilus assembly protein PilG Machinery gene
  G4V50_RS08715 pilD 1686017..1686877 (+) 861 WP_003689814.1 prepilin peptidase Machinery gene
  G4V50_RS08720 coaE 1686874..1687506 (+) 633 WP_003689816.1 dephospho-CoA kinase -
  G4V50_RS08725 - 1687499..1687708 (+) 210 WP_003696096.1 DNA gyrase inhibitor YacG -
  G4V50_RS08730 pilF 1687794..1689470 (-) 1677 WP_003689817.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G4V50_RS08740 - 1689808..1690254 (-) 447 WP_003689818.1 DUF441 domain-containing protein -
  G4V50_RS08745 - 1690258..1691232 (-) 975 WP_003689819.1 polyprenyl synthetase family protein -
  G4V50_RS08755 rplU 1691456..1691764 (+) 309 WP_003691838.1 50S ribosomal protein L21 -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31332.34 Da        Isoelectric Point: 8.9561

>NTDB_id=423918 G4V50_RS08715 WP_003689814.1 1686017..1686877(+) (pilD) [Neisseria gonorrhoeae strain SRRSH214]
MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVFAKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVR

Nucleotide


Download         Length: 861 bp        

>NTDB_id=423918 G4V50_RS08715 WP_003689814.1 1686017..1686877(+) (pilD) [Neisseria gonorrhoeae strain SRRSH214]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCAGTTTTGGGGCTGCTGGTCGGCAGCTTCCTGAA
TGTCGTCATTTACCGCGTACCCGTTATGATGGAACGCGGCTGGACGGTATTTGCCAAAGAACATTTAAACCTGCCGCTGA
CCGACGATGAAAGCCGTACCTTCAACCTGATGAAGCCGGATTCCTGCTGTCCCAAATGCCGTGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGTTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTGTTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACGCTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACATTACCC
TTGATCTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATAGTTCATTATGGCTCTTATGTGCAGTGTATAAACTGCTCACAGGAAAAACCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCTCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCGGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGAGATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(134-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

100

100

1
  pilD Vibrio cholerae strain A1552

44.792

100

0.451
  pilD Vibrio campbellii strain DS40M4

45.652

96.503

0.441
  pilD Acinetobacter baumannii D1279779

47.727

92.308

0.441
  pilD Acinetobacter nosocomialis M2

47.727

92.308

0.441