Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   IEM32_RS24490 Genome accession   NZ_CP062160
Coordinates   4959200..4959859 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 20R2R     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4954200..4964859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEM32_RS24475 (IEM32_24475) ygiS 4956405..4958012 (-) 1608 WP_001301989.1 ABC transporter substrate-binding protein -
  IEM32_RS24480 (IEM32_24480) ygiV 4958121..4958603 (-) 483 WP_000183479.1 GyrI-like domain-containing protein -
  IEM32_RS24485 (IEM32_24485) ygiW 4958656..4959048 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  IEM32_RS24490 (IEM32_24490) ciaR 4959200..4959859 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  IEM32_RS24495 (IEM32_24495) qseC 4959856..4961205 (+) 1350 WP_000673362.1 quorum sensing histidine kinase QseC -
  IEM32_RS24500 (IEM32_24500) mdaB 4961315..4961896 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  IEM32_RS24505 (IEM32_24505) ygiN 4961927..4962241 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  IEM32_RS24510 (IEM32_24510) - 4962286..4963173 (-) 888 WP_000614950.1 MurR/RpiR family transcriptional regulator -
  IEM32_RS24515 (IEM32_24515) - 4963170..4964117 (-) 948 WP_001301797.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=423894 IEM32_RS24490 WP_001221502.1 4959200..4959859(+) (ciaR) [Escherichia coli strain 20R2R]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=423894 IEM32_RS24490 WP_001221502.1 4959200..4959859(+) (ciaR) [Escherichia coli strain 20R2R]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365