Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   IE857_RS03545 Genome accession   NZ_CP061914
Coordinates   730333..730992 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain SMBL     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 725333..735992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IE857_RS03520 (IE857_03520) - 725990..727321 (+) 1332 WP_000778880.1 MFS transporter -
  IE857_RS03525 (IE857_03525) ygiN 727365..727679 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  IE857_RS03530 (IE857_03530) mdaB 727710..728290 (-) 581 Protein_699 NADPH:quinone oxidoreductase MdaB -
  IE857_RS03535 (IE857_03535) ygiZ 728609..728941 (+) 333 WP_000912120.1 DUF2645 family protein -
  IE857_RS03540 (IE857_03540) qseC 728987..730336 (-) 1350 WP_033547759.1 quorum sensing histidine kinase QseC -
  IE857_RS03545 (IE857_03545) ciaR 730333..730992 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  IE857_RS03550 (IE857_03550) ygiW 731144..731536 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  IE857_RS03555 (IE857_03555) ygiV 731589..732071 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  IE857_RS03560 (IE857_03560) parC 732617..734875 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  IE857_RS03565 (IE857_03565) plsC 735109..735846 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=423583 IE857_RS03545 WP_001221502.1 730333..730992(-) (ciaR) [Escherichia coli strain SMBL]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=423583 IE857_RS03545 WP_001221502.1 730333..730992(-) (ciaR) [Escherichia coli strain SMBL]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365