Detailed information NTG0423577

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: G4P54_RS13430
• Genome accession: NZ_CP048852
• Coordinates: 2512609..2513226 (-)
• Length: 205 a.a.
• NCBI ID: WP_167872908.1
• Uniprot ID: -
• Organism: Bacillus tequilensis strain EA-CB0015
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2507609..2518226

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G4P54_RS13400 (G4P54_13355)	rpsT	2507910..2508176 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
G4P54_RS13405 (G4P54_13360)	holA	2508191..2509234 (-)	1044	WP_024714992.1	DNA polymerase III subunit delta	-
G4P54_RS13410 (G4P54_13365)	-	2509274..2509423 (-)	150	WP_167872907.1	hypothetical protein	-
G4P54_RS13415 (G4P54_13370)	-	2509464..2509598 (+)	135	WP_003229983.1	YqzM family protein	-
G4P54_RS13420 (G4P54_13375)	comEC	2509639..2511969 (-)	2331	WP_167873880.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
G4P54_RS13425 (G4P54_13380)	-	2511972..2512541 (-)	570	WP_024714990.1	ComE operon protein 2	-
G4P54_RS13430 (G4P54_13385)	comEA	2512609..2513226 (-)	618	WP_167872908.1	helix-hairpin-helix domain-containing protein	Machinery gene
G4P54_RS13435 (G4P54_13390)	comER	2513310..2514131 (+)	822	WP_167872909.1	late competence protein ComER	-
G4P54_RS13440 (G4P54_13395)	-	2514197..2514940 (-)	744	WP_167872910.1	class I SAM-dependent DNA methyltransferase	-
G4P54_RS13445 (G4P54_13400)	rsfS	2514937..2515293 (-)	357	WP_167872911.1	ribosome silencing factor	-
G4P54_RS13450 (G4P54_13405)	yqeK	2515311..2515871 (-)	561	WP_167872912.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
G4P54_RS13455 (G4P54_13410)	-	2515861..2516430 (-)	570	WP_024714985.1	nicotinate-nucleotide adenylyltransferase	-
G4P54_RS13460 (G4P54_13415)	yhbY	2516442..2516732 (-)	291	WP_024714984.1	ribosome assembly RNA-binding protein YhbY	-
G4P54_RS13465 (G4P54_13420)	aroE	2516726..2517568 (-)	843	WP_167872913.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21928.81 Da
• Isoelectric Point: 4.7544

>NTDB_id=423577 G4P54_RS13430 WP_167872908.1 2512609..2513226(-) (comEA) [Bacillus tequilensis strain EA-CB0015]
MNWLNQHKTVIILTASAAVVITIIFFYAIGKNKEPVEHAVSTETANPDVKQEAAKDESKETIVIDIKGAVQRPGVYEMRT
GDRVSQAIEKAGGTSEQADELQVNLAELLQDGTVVYIPKKGEEKAAQQGAGVAVKSGGGEGALVNINTATIEELQDISGV
GPSKAEAIVAYREENGRFQTIEDIMKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=423577 G4P54_RS13430 WP_167872908.1 2512609..2513226(-) (comEA) [Bacillus tequilensis strain EA-CB0015]
ATGAATTGGTTGAATCAGCATAAGACAGTAATTATTTTAACTGCTTCTGCGGCTGTTGTCATAACGATTATTTTCTTCTA
TGCCATCGGAAAAAACAAAGAGCCGGTGGAACACGCTGTATCAACTGAAACAGCAAATCCAGACGTAAAGCAGGAGGCGG
CCAAAGACGAGTCAAAGGAAACAATTGTGATTGACATCAAAGGTGCTGTTCAGCGTCCCGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGAGGGACAAGTGAACAAGCAGACGAACTGCAAGTGAATTTGGCAGA
GCTGCTGCAGGACGGGACAGTTGTGTATATTCCGAAAAAGGGAGAGGAGAAAGCGGCACAGCAAGGAGCCGGGGTTGCTG
TCAAAAGCGGTGGAGGGGAGGGAGCGCTGGTCAATATCAATACAGCAACTATAGAGGAATTACAAGATATCTCAGGGGTC
GGGCCATCCAAAGCTGAAGCTATTGTTGCCTATCGGGAAGAAAACGGGCGTTTCCAGACAATAGAAGATATTATGAAGGT
TTCAGGAATAGGCGAAAAGTCGTTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (143-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	83.415	100	0.834
comEA	Staphylococcus aureus N315	37.727	100	0.405
comEA	Staphylococcus aureus MW2	36.818	100	0.395
comEA	Latilactobacillus sakei subsp. sakei 23K	34.884	100	0.366