Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   IE382_RS17165 Genome accession   NZ_CP061870
Coordinates   3140590..3141594 (-) Length   334 a.a.
NCBI ID   WP_017696349.1    Uniprot ID   -
Organism   Bacillus subtilis strain FX-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3135590..3146594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IE382_RS17135 (IE382_17050) yrbG 3136408..3137064 (-) 657 WP_017696351.1 DUF421 domain-containing protein -
  IE382_RS17140 (IE382_17055) - 3137262..3137651 (+) 390 WP_029727131.1 TIGR04086 family membrane protein -
  IE382_RS17145 (IE382_17060) yajC 3137863..3138129 (-) 267 WP_004398708.1 preprotein translocase subunit YajC -
  IE382_RS17150 (IE382_17065) tgt 3138167..3139312 (-) 1146 WP_003229725.1 tRNA guanosine(34) transglycosylase Tgt -
  IE382_RS17155 (IE382_17070) queA 3139339..3140367 (-) 1029 WP_014477530.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  IE382_RS17160 (IE382_17075) yrzS 3140397..3140597 (-) 201 WP_003222669.1 DUF2905 domain-containing protein -
  IE382_RS17165 (IE382_17080) ruvB 3140590..3141594 (-) 1005 WP_017696349.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  IE382_RS17170 (IE382_17085) ruvA 3141605..3142210 (-) 606 WP_144458755.1 Holliday junction branch migration protein RuvA Machinery gene
  IE382_RS17175 (IE382_17090) bofC 3142349..3142861 (-) 513 WP_003229715.1 sporulation cell-cell signaling protein BofC -
  IE382_RS17180 (IE382_17095) csbX 3142909..3144216 (-) 1308 WP_003246197.1 MFS transporter -
  IE382_RS17185 (IE382_17100) iolG 3144287..3145312 (-) 1026 WP_004398990.1 inositol 2-dehydrogenase -
  IE382_RS17190 (IE382_17105) yrzF 3145550..3146197 (+) 648 WP_003246159.1 phosphotransferase -
  IE382_RS17195 (IE382_17110) - 3146243..3146365 (-) 123 WP_003229707.1 hypothetical protein -
  IE382_RS17200 - 3146450..3146524 (+) 75 Protein_3351 hypothetical protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37483.78 Da        Isoelectric Point: 5.1631

>NTDB_id=423534 IE382_RS17165 WP_017696349.1 3140590..3141594(-) (ruvB) [Bacillus subtilis strain FX-1]
MDERLVSSEADNHESVIEQSLRPQNLSQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=423534 IE382_RS17165 WP_017696349.1 3140590..3141594(-) (ruvB) [Bacillus subtilis strain FX-1]
ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGTC
ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG
ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACTACGCTTGCTTCGATTGTTGCCAATGAAATGGGAGTGGAG
CTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT
ATTATTTATTGATGAAATACATAGGTTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG
ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGGCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACA
AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTCATGTCTCGCCTTGAGTACTATACACAAGAAGAACT
GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT
CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGTGATTTCGCCCAAGTGCTGGGTGACAGCAGGATC
ACTGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGATCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATATCAGCTACGATCGGCGAGGAATCGCATA
CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG
GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB AAA lid domain (AAA_lid_4)

(182-255)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

99.701

100

0.997
  ruvB Streptococcus pneumoniae R6

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae D39

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae TIGR4

60.429

97.605

0.59
  ruvB Synechocystis sp. PCC 6803

56.839

98.503

0.56
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518