Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IE752_RS26470 Genome accession   NZ_CP061850
Coordinates   5669804..5670427 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain R31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5664804..5675427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IE752_RS26440 (IE752_26440) - 5665803..5666012 (-) 210 WP_191519356.1 hypothetical protein -
  IE752_RS26445 (IE752_26445) - 5666012..5666446 (-) 435 WP_015984360.1 hypothetical protein -
  IE752_RS26450 (IE752_26450) - 5666449..5666793 (-) 345 WP_034065298.1 helix-turn-helix domain-containing protein -
  IE752_RS26455 (IE752_26455) - 5666943..5667671 (+) 729 WP_079389759.1 XRE family transcriptional regulator -
  IE752_RS26460 (IE752_26460) pilQ 5667841..5669229 (-) 1389 Protein_5249 type 4a pilus secretin PilQ -
  IE752_RS26465 (IE752_26465) comL/pilP 5669283..5669807 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  IE752_RS26470 (IE752_26470) pilO 5669804..5670427 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  IE752_RS26475 (IE752_26475) pilN 5670424..5671020 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  IE752_RS26480 (IE752_26480) pilM 5671020..5672084 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  IE752_RS26485 (IE752_26485) - 5672269..5674740 (+) 2472 WP_031756300.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=423083 IE752_RS26470 WP_003103268.1 5669804..5670427(-) (pilO) [Pseudomonas aeruginosa strain R31]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=423083 IE752_RS26470 WP_003103268.1 5669804..5670427(-) (pilO) [Pseudomonas aeruginosa strain R31]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401