Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LYSZa7_RS19355 Genome accession   NZ_CP061699
Coordinates   4233775..4235502 (-) Length   575 a.a.
NCBI ID   WP_200199799.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain LYSZa7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4228775..4240502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LYSZa7_RS19325 (LYSZa7_19240) - 4229635..4230714 (-) 1080 WP_003131071.1 general secretion pathway protein GspK -
  LYSZa7_RS19330 (LYSZa7_19245) - 4230704..4231294 (-) 591 WP_016852732.1 type II secretion system protein J -
  LYSZa7_RS19335 (LYSZa7_19250) - 4231291..4231716 (-) 426 WP_003110087.1 type II secretion system protein J -
  LYSZa7_RS19340 (LYSZa7_19255) - 4231716..4232126 (-) 411 WP_003097723.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LYSZa7_RS19345 (LYSZa7_19260) gspG 4232095..4232529 (-) 435 WP_022579956.1 type II secretion system major pseudopilin GspG -
  LYSZa7_RS19350 (LYSZa7_19265) - 4232583..4233770 (-) 1188 WP_003110091.1 type II secretion system F family protein -
  LYSZa7_RS19355 (LYSZa7_19270) pilF 4233775..4235502 (-) 1728 WP_200199799.1 GspE/PulE family protein Machinery gene
  LYSZa7_RS19360 (LYSZa7_19275) - 4235492..4236295 (-) 804 WP_003131067.1 ABC transporter permease -
  LYSZa7_RS19365 (LYSZa7_19280) - 4236739..4237701 (-) 963 WP_023094659.1 GlxA family transcriptional regulator -
  LYSZa7_RS19370 (LYSZa7_19285) - 4237879..4238670 (+) 792 WP_200199800.1 alpha/beta fold hydrolase -
  LYSZa7_RS19375 (LYSZa7_19290) - 4238694..4239446 (-) 753 WP_003110095.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63697.73 Da        Isoelectric Point: 5.1633

>NTDB_id=422170 LYSZa7_RS19355 WP_200199799.1 4233775..4235502(-) (pilF) [Pseudomonas aeruginosa strain LYSZa7]
MSSEAVSPGPQVTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGDDL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFATRDPLSPSIRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LARQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLRLDRLGMLPDHLQRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLISVEDPVEYQVPNITQVQVHADIGLTFAAALRSILRQDPDVIMIGEI
RDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAA
TEAATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAW
LGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=422170 LYSZa7_RS19355 WP_200199799.1 4233775..4235502(-) (pilF) [Pseudomonas aeruginosa strain LYSZa7]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGGTGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGCCTGGGAGGTATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGCTGGTGGGGGACGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGCACCCCGATCAATACCGACTGGTTCGTCGAAGAGCAACT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGACGCGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCAGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCATTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCACGCCTATGGCAGCGAAGACATCCGCTACCTGCGGGAGCTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGCTCTTCGGATATTCACATCGAACCCCGGGAAT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTGCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTT
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTAGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTCATGCGCCTGTTGCCGA
AGGAGCGCGAGGGATTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCAGAGGATGCGTGCATGGGTAGCGGAA
CCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCCACCACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTGGAATACCAGGTGCCCAACATCACCCAGGTCCAGGTCCATG
CCGACATCGGCCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATA
CGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGA
TGCGGTGAGTGCCTTCACGCGCTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGG
CCCAGCGACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG
ACCGAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTGCGAAGG
TACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGAG
CTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGG
TTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(194-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

41.696

98.435

0.41
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.801

98.609

0.383
  pilF Neisseria gonorrhoeae MS11

36.842

99.13

0.365
  pilB Acinetobacter baumannii D1279779

35.135

100

0.362