Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   GZ067_RS02345 Genome accession   NZ_CP048431
Coordinates   445736..446233 (+) Length   165 a.a.
NCBI ID   WP_029694050.1    Uniprot ID   -
Organism   Staphylococcus aureus strain SA1428     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 440736..451233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZ067_RS02305 (GZ067_02305) - 440780..441766 (+) 987 WP_000161314.1 ROK family glucokinase -
  GZ067_RS02310 (GZ067_02310) - 441766..442095 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  GZ067_RS02315 (GZ067_02315) - 442092..442715 (+) 624 WP_117223063.1 MBL fold metallo-hydrolase -
  GZ067_RS02320 (GZ067_02320) comGA 442767..443741 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  GZ067_RS02325 (GZ067_02325) comGB 443713..444783 (+) 1071 WP_000776421.1 competence type IV pilus assembly protein ComGB Machinery gene
  GZ067_RS02330 (GZ067_02330) comGC 444797..445108 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  GZ067_RS02335 (GZ067_02335) comGD 445086..445532 (+) 447 WP_072433556.1 competence type IV pilus minor pilin ComGD Machinery gene
  GZ067_RS02340 (GZ067_02340) comGE 445519..445818 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  GZ067_RS02345 (GZ067_02345) comGF 445736..446233 (+) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  GZ067_RS02350 (GZ067_02350) - 446330..446476 (+) 147 WP_001789879.1 hypothetical protein -
  GZ067_RS02355 (GZ067_02355) - 446466..446990 (+) 525 WP_001015121.1 shikimate kinase -
  GZ067_RS02360 (GZ067_02360) gcvT 447149..448240 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  GZ067_RS02365 (GZ067_02365) gcvPA 448260..449606 (+) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  GZ067_RS02370 (GZ067_02370) gcvPB 449599..451071 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19199.92 Da        Isoelectric Point: 10.2192

>NTDB_id=421294 GZ067_RS02345 WP_029694050.1 445736..446233(+) (comGF) [Staphylococcus aureus strain SA1428]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIELRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTSFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=421294 GZ067_RS02345 WP_029694050.1 445736..446233(+) (comGF) [Staphylococcus aureus strain SA1428]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAACTTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CTTCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

96.97

100

0.97
  comGF Staphylococcus aureus N315

96.97

100

0.97

Multiple sequence alignment