Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   D8K17_RS11435 Genome accession   NZ_CP061322
Coordinates   2247020..2247466 (-) Length   148 a.a.
NCBI ID   WP_031296844.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis bv. diacetylactis strain S50     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2242020..2252466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8K17_RS11400 (D8K17_011420) - 2242137..2242514 (-) 378 WP_003129983.1 pyridoxamine 5'-phosphate oxidase family protein -
  D8K17_RS11405 (D8K17_011425) - 2242719..2243585 (+) 867 WP_010906309.1 RluA family pseudouridine synthase -
  D8K17_RS11410 (D8K17_011430) - 2243624..2244433 (-) 810 WP_010906310.1 metal ABC transporter permease -
  D8K17_RS11415 (D8K17_011435) - 2244426..2245163 (-) 738 WP_010906311.1 metal ABC transporter ATP-binding protein -
  D8K17_RS11420 (D8K17_011440) - 2245340..2246182 (-) 843 WP_010906312.1 metal ABC transporter substrate-binding protein -
  D8K17_RS11425 (D8K17_011445) - 2246179..2246616 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  D8K17_RS11430 (D8K17_011450) comGG 2246697..2246981 (-) 285 WP_010906314.1 competence type IV pilus minor pilin ComGG Machinery gene
  D8K17_RS11435 (D8K17_011455) comGF 2247020..2247466 (-) 447 WP_031296844.1 competence type IV pilus minor pilin ComGF Machinery gene
  D8K17_RS11440 (D8K17_011460) comGE 2247429..2247725 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  D8K17_RS11445 (D8K17_011465) comGD 2247697..2248113 (-) 417 WP_021216554.1 competence type IV pilus minor pilin ComGD Machinery gene
  D8K17_RS11450 (D8K17_011470) comGC 2248088..2248357 (-) 270 WP_023349160.1 competence type IV pilus major pilin ComGC Machinery gene
  D8K17_RS11455 (D8K17_011475) - 2248504..2249028 (-) 525 WP_014570795.1 GNAT family N-acetyltransferase -
  D8K17_RS11460 (D8K17_011480) - 2249107..2249886 (-) 780 WP_058147788.1 peptidoglycan amidohydrolase family protein -
  D8K17_RS11465 (D8K17_011485) - 2249886..2250185 (-) 300 WP_031559226.1 phage holin -
  D8K17_RS11470 (D8K17_011490) - 2250198..2250548 (-) 351 WP_023349296.1 hypothetical protein -
  D8K17_RS14055 - 2250568..2252148 (-) 1581 WP_223203828.1 hypothetical protein -
  D8K17_RS11480 (D8K17_011500) - 2252126..2252299 (-) 174 WP_032941620.1 hypothetical protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 17083.85 Da        Isoelectric Point: 8.6780

>NTDB_id=421091 D8K17_RS11435 WP_031296844.1 2247020..2247466(-) (comGF) [Lactococcus lactis subsp. lactis bv. diacetylactis strain S50]
MERKLCDLNFKVKAFTLLECLVALLAISGSALVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide


Download         Length: 447 bp        

>NTDB_id=421091 D8K17_RS11435 WP_031296844.1 2247020..2247466(-) (comGF) [Lactococcus lactis subsp. lactis bv. diacetylactis strain S50]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGCCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(48-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

75.54

93.919

0.709
  comGF Streptococcus mutans UA140

47.518

95.27

0.453
  comGF Streptococcus mutans UA159

46.809

95.27

0.446
  comGF/cglF Streptococcus mitis SK321

43.066

92.568

0.399
  comGF/cglF Streptococcus pneumoniae Rx1

40.426

95.27

0.385
  comGF/cglF Streptococcus pneumoniae D39

40.426

95.27

0.385
  comGF/cglF Streptococcus pneumoniae R6

40.426

95.27

0.385
  comGF/cglF Streptococcus pneumoniae TIGR4

40.426

95.27

0.385
  comGF/cglF Streptococcus mitis NCTC 12261

41.606

92.568

0.385