Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M654_RS23415 Genome accession   NZ_CP048273
Coordinates   4537012..4538391 (-) Length   459 a.a.
NCBI ID   WP_026586091.1    Uniprot ID   A0A6I7FJ42
Organism   Bacillus sp. NSP9.1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4532012..4543391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M654_RS23390 (M654_023320) gltX 4532021..4533478 (-) 1458 WP_026586086.1 glutamate--tRNA ligase -
  M654_RS23395 (M654_023325) ispF 4533558..4534034 (-) 477 WP_026586087.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  M654_RS23400 (M654_023330) ispD 4534027..4534722 (-) 696 WP_026586088.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  M654_RS23405 (M654_023335) - 4534737..4535825 (-) 1089 WP_026586089.1 PIN/TRAM domain-containing protein -
  M654_RS23410 (M654_023340) disA 4535932..4537008 (-) 1077 WP_026586090.1 DNA integrity scanning diadenylate cyclase DisA -
  M654_RS23415 (M654_023345) radA 4537012..4538391 (-) 1380 WP_026586091.1 DNA repair protein RadA Machinery gene
  M654_RS23420 (M654_023350) clpC 4538469..4540901 (-) 2433 WP_026586092.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  M654_RS23425 (M654_023355) - 4540898..4541989 (-) 1092 WP_026586093.1 protein arginine kinase -
  M654_RS23430 (M654_023360) - 4541989..4542549 (-) 561 WP_026586094.1 UvrB/UvrC motif-containing protein -
  M654_RS23435 (M654_023365) - 4542564..4543028 (-) 465 WP_026586095.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49997.57 Da        Isoelectric Point: 7.6840

>NTDB_id=420481 M654_RS23415 WP_026586091.1 4537012..4538391(-) (radA) [Bacillus sp. NSP9.1]
MAKTKSKFICQSCGYESAKWMGKCPGCGAWNTMVEEMIKKQAANRRTAFSHSIQKVQKPSPITSIETTEEPRIQTKLREF
NRVLGNGIVKGSLVLIGGDPGIGKSTLLLQVSAQLADLKENVLYISGEESVKQTKLRADRLGINSPTLHVLSETDMEYIT
SSIEEMNPSFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMRIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGAAGSSVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAISIASSFRDAPPQPTDCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMIIPSANVEGWTKPKGIEVVGVENVAEALRASLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=420481 M654_RS23415 WP_026586091.1 4537012..4538391(-) (radA) [Bacillus sp. NSP9.1]
ATGGCTAAAACGAAAAGTAAATTTATTTGTCAATCATGCGGCTATGAATCAGCCAAATGGATGGGGAAGTGTCCGGGATG
CGGCGCATGGAACACCATGGTCGAAGAAATGATCAAAAAGCAGGCCGCTAACAGAAGAACCGCCTTTTCACATTCCATCC
AAAAGGTGCAAAAGCCATCCCCCATTACATCAATTGAAACGACGGAAGAGCCGAGGATCCAAACGAAATTGCGCGAGTTC
AACCGCGTTTTGGGAAATGGCATCGTCAAGGGATCGCTCGTGTTAATCGGCGGTGACCCAGGTATCGGAAAATCCACTTT
GCTGCTGCAGGTGTCTGCCCAGCTTGCTGATTTGAAGGAAAATGTCCTGTACATATCAGGGGAAGAATCGGTCAAACAAA
CAAAGCTGAGAGCGGACCGCCTCGGCATCAACAGTCCAACTCTTCACGTTTTATCTGAAACCGATATGGAGTATATTACG
TCTTCTATAGAAGAGATGAACCCTTCATTTGTGGTGGTAGACTCCATTCAAACCGTTTACCAAAGCGATATTACATCAGC
CCCGGGCAGCGTATCCCAGGTAAGGGAATGCACCGCTGAGCTGATGAGAATTGCCAAAACAAAAGGGATTCCAATATTTA
TCGTCGGCCATGTCACAAAAGAGGGCTCGATTGCCGGCCCGAGACTGCTTGAGCACATGGTCGACACCGTTCTTTATTTT
GAAGGAGAACGCCATCACACATTCCGGATTTTAAGAGCTGTTAAAAACAGGTTTGGATCGACAAACGAGATGGGGATTTT
TGAAATGAGAGAAGAAGGCTTGACAGAGGTGCTGAATCCGTCGGAAATTTTTCTCGAAGAACGTTCGGCAGGTGCCGCCG
GGTCAAGCGTTGTCGCCTCAATGGAAGGCACTAGACCGGTTCTGGTCGAAATTCAAGCGCTGATCTCACCTACCAGCTTC
GGGAATCCGCGCAGAATGGCTACCGGAATCGATCATAATCGCGTCTCCCTGCTCATGGCGGTTCTCGAAAAAAGAGTGGG
TCTCCTGCTGCAAAATCAAGATGCATATTTAAAAGTTGCCGGCGGAGTGAAGCTGGATGAGCCCGCGATCGACCTTGCCG
TTGCAATCAGTATCGCGTCAAGCTTCAGGGATGCTCCGCCTCAACCGACAGATTGCTTTATCGGCGAGGTCGGCCTTACG
GGAGAGGTCCGCAGAGTATCAAGAATTGAACAGAGGGTTCAAGAAGCCGCAAAGCTTGGTTTCAAAAGAATGATTATCCC
TTCTGCCAATGTGGAAGGCTGGACCAAGCCGAAGGGAATTGAGGTCGTCGGTGTTGAAAATGTTGCTGAGGCCCTTCGAG
CTTCATTAGGAGGATCATGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I7FJ42

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

91.048

99.782

0.908
  radA Streptococcus pneumoniae Rx1

62.252

98.693

0.614
  radA Streptococcus pneumoniae D39

62.252

98.693

0.614
  radA Streptococcus pneumoniae R6

62.252

98.693

0.614
  radA Streptococcus pneumoniae TIGR4

62.252

98.693

0.614
  radA Streptococcus mitis NCTC 12261

62.252

98.693

0.614
  radA Streptococcus mitis SK321

62.031

98.693

0.612

Multiple sequence alignment