Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   SPPN_RS11160 Genome accession   NC_015875
Coordinates   2158519..2159817 (-) Length   432 a.a.
NCBI ID   WP_000867589.1    Uniprot ID   -
Organism   Streptococcus pseudopneumoniae IS7493     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2153519..2164817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPPN_RS11140 (SPPN_11230) rplI 2154674..2155126 (-) 453 WP_000864214.1 50S ribosomal protein L9 -
  SPPN_RS11145 (SPPN_11235) - 2155123..2157096 (-) 1974 WP_000715117.1 DHH family phosphoesterase -
  SPPN_RS11150 (SPPN_11240) hpf 2157232..2157780 (-) 549 WP_000599099.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SPPN_RS11155 (SPPN_11245) comFC/cflB 2157860..2158522 (-) 663 WP_000649947.1 ComF family protein Machinery gene
  SPPN_RS11160 (SPPN_11250) comFA/cflA 2158519..2159817 (-) 1299 WP_000867589.1 DEAD/DEAH box helicase Machinery gene
  SPPN_RS11165 (SPPN_11255) - 2159873..2160508 (+) 636 WP_000395743.1 YigZ family protein -
  SPPN_RS14110 - 2160613..2160696 (+) 84 Protein_2224 PH domain-containing protein -
  SPPN_RS11170 (SPPN_11260) cysK 2160793..2161719 (+) 927 WP_000029891.1 cysteine synthase A -
  SPPN_RS11175 (SPPN_11265) tsf 2161841..2162881 (-) 1041 WP_000808091.1 translation elongation factor Ts -
  SPPN_RS11180 (SPPN_11270) rpsB 2162961..2163740 (-) 780 WP_014017378.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49389.38 Da        Isoelectric Point: 8.6608

>NTDB_id=42045 SPPN_RS11160 WP_000867589.1 2158519..2159817(-) (comFA/cflA) [Streptococcus pseudopneumoniae IS7493]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKDKGKLVCQRCNSAILEEWYLPIGAYYCRECLIMKRVRSDQALYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINGGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSDFNRYLDKKCLSPKLKSYIDKQRKTGYPLLIFASEIKKGEQLKEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNTSIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=42045 SPPN_RS11160 WP_000867589.1 2158519..2159817(-) (comFA/cflA) [Streptococcus pseudopneumoniae IS7493]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAGGAGCGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGATAAGGGGAAACTTGTCTGTCAACGTTGTAATAGTGCTATTCTGGAGGAATGGTATTTGC
CCATTGGTGCTTACTATTGTCGGGAGTGCTTAATTATGAAGCGAGTCAGGAGTGATCAAGCTTTATACTATTTCCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTACT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATTAATGGAGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTATAAGCGC
CTGCAACAGGATTTTTCTTGCGGAATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
GACGACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGTTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGATAAGAAGTGTTTGTCGCCAAAGTTAAAGTCCTATA
TTGATAAGCAGAGAAAGACAGGTTATCCGTTACTTATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAAAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGGGAGCTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACATTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAAATTGGTGGACGAGTTGGACGAAGTATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATACTTCAATTAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(292-396)

DEAD/DEAH box helicase (DEAD)

(96-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

96.759

100

0.968
  comFA/cflA Streptococcus pneumoniae D39

96.759

100

0.968
  comFA/cflA Streptococcus pneumoniae R6

96.759

100

0.968
  comFA/cflA Streptococcus pneumoniae TIGR4

96.528

100

0.965
  comFA/cflA Streptococcus mitis SK321

92.593

100

0.926
  comFA/cflA Streptococcus mitis NCTC 12261

92.593

100

0.926
  comFA Lactococcus lactis subsp. cremoris KW2

51.961

94.444

0.491
  comFA Latilactobacillus sakei subsp. sakei 23K

38.391

100

0.387

Multiple sequence alignment