Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   IB009_RS03585 Genome accession   NZ_CP061101
Coordinates   743174..743833 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 1EC213     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 738174..748833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IB009_RS03560 (IB009_03555) parE 738265..740157 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  IB009_RS03565 (IB009_03560) ygiN 740205..740519 (-) 315 WP_074684072.1 putative quinol monooxygenase -
  IB009_RS03570 (IB009_03565) mdaB 740550..741131 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  IB009_RS03575 (IB009_03570) ygiZ 741450..741782 (+) 333 WP_000912120.1 DUF2645 family protein -
  IB009_RS03580 (IB009_03575) qseC 741828..743177 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  IB009_RS03585 (IB009_03580) ciaR 743174..743833 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  IB009_RS03590 (IB009_03585) ygiW 743985..744377 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  IB009_RS03595 (IB009_03590) ygiV 744430..744912 (+) 483 WP_000183481.1 GyrI-like domain-containing protein -
  IB009_RS03600 (IB009_03595) parC 745458..747716 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  IB009_RS03605 (IB009_03600) plsC 747949..748686 (+) 738 WP_000965703.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=420052 IB009_RS03585 WP_001221502.1 743174..743833(-) (ciaR) [Escherichia coli strain 1EC213]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=420052 IB009_RS03585 WP_001221502.1 743174..743833(-) (ciaR) [Escherichia coli strain 1EC213]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365