Detailed information NTG0419209

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: GWK37_RS01965
• Genome accession: NZ_CP048002
• Coordinates: 393912..394526 (-)
• Length: 204 a.a.
• NCBI ID: WP_115940650.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain CACC 316
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 388912..399526

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GWK37_RS01935 (GWK37_01940)	rpsT	389215..389481 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
GWK37_RS01940 (GWK37_01945)	holA	389498..390539 (-)	1042	Protein_385	DNA polymerase III subunit delta	-
GWK37_RS01945 (GWK37_01950)	-	390579..390730 (-)	152	Protein_386	hypothetical protein	-
GWK37_RS01950 (GWK37_01955)	-	390771..390905 (+)	135	WP_003152870.1	YqzM family protein	-
GWK37_RS01955 (GWK37_01960)	comEC	390924..393275 (-)	2352	WP_115940649.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GWK37_RS01960 (GWK37_01965)	-	393276..393845 (-)	570	WP_003152868.1	ComE operon protein 2	-
GWK37_RS01965 (GWK37_01970)	comEA	393912..394526 (-)	615	WP_115940650.1	helix-hairpin-helix domain-containing protein	Machinery gene
GWK37_RS01970 (GWK37_01975)	comER	394585..395406 (+)	822	WP_012118027.1	late competence protein ComER	-
GWK37_RS01975 (GWK37_01980)	-	395475..396212 (-)	738	WP_015417856.1	class I SAM-dependent DNA methyltransferase	-
GWK37_RS01980 (GWK37_01985)	rsfS	396209..396565 (-)	357	WP_007408260.1	ribosome silencing factor	-
GWK37_RS01985 (GWK37_01990)	yqeK	396583..397143 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
GWK37_RS01990 (GWK37_01995)	-	397133..397702 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
GWK37_RS01995 (GWK37_02000)	yhbY	397713..398003 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
GWK37_RS02000 (GWK37_02005)	aroE	397997..398839 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22225.28 Da
• Isoelectric Point: 5.2093

>NTDB_id=419209 GWK37_RS01965 WP_115940650.1 393912..394526(-) (comEA) [Bacillus velezensis strain CACC 316]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSENEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=419209 GWK37_RS01965 WP_115940650.1 393912..394526(-) (comEA) [Bacillus velezensis strain CACC 316]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAACGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGATTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363