Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GWK37_RS01540 Genome accession   NZ_CP048002
Coordinates   317399..318469 (-) Length   356 a.a.
NCBI ID   WP_014418378.1    Uniprot ID   A0AAP4DGV9
Organism   Bacillus velezensis strain CACC 316     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 312399..323469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWK37_RS01490 (GWK37_01495) sipW 312458..313042 (-) 585 WP_015240205.1 signal peptidase I SipW -
  GWK37_RS01495 (GWK37_01500) tapA 313014..313685 (-) 672 WP_025649852.1 amyloid fiber anchoring/assembly protein TapA -
  GWK37_RS01500 (GWK37_01505) - 313944..314273 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  GWK37_RS01505 (GWK37_01510) - 314314..314493 (-) 180 WP_003153093.1 YqzE family protein -
  GWK37_RS01510 (GWK37_01515) comGG 314550..314927 (-) 378 WP_017418138.1 competence type IV pilus minor pilin ComGG Machinery gene
  GWK37_RS01515 (GWK37_01520) comGF 314928..315323 (-) 396 WP_115940643.1 competence type IV pilus minor pilin ComGF -
  GWK37_RS01520 (GWK37_01525) comGE 315337..315651 (-) 315 WP_029973875.1 competence type IV pilus minor pilin ComGE Machinery gene
  GWK37_RS01525 (GWK37_01530) comGD 315635..316072 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  GWK37_RS01530 (GWK37_01535) comGC 316062..316328 (-) 267 WP_050496152.1 competence type IV pilus major pilin ComGC Machinery gene
  GWK37_RS01535 (GWK37_01540) comGB 316375..317412 (-) 1038 WP_014418377.1 competence type IV pilus assembly protein ComGB Machinery gene
  GWK37_RS01540 (GWK37_01545) comGA 317399..318469 (-) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  GWK37_RS01545 (GWK37_01550) - 318662..319612 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  GWK37_RS01550 (GWK37_01555) - 319758..321059 (+) 1302 WP_029973873.1 hemolysin family protein -
  GWK37_RS01555 (GWK37_01560) - 321097..321927 (-) 831 WP_025649847.1 STAS domain-containing protein -
  GWK37_RS01565 (GWK37_01570) - 322134..322514 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  GWK37_RS01570 (GWK37_01575) - 322751..322996 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=419205 GWK37_RS01540 WP_014418378.1 317399..318469(-) (comGA) [Bacillus velezensis strain CACC 316]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=419205 GWK37_RS01540 WP_014418378.1 317399..318469(-) (comGA) [Bacillus velezensis strain CACC 316]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTAAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGTGCCGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment