Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   M594_RS09860 Genome accession   NZ_CP047883
Coordinates   2054445..2055743 (-) Length   432 a.a.
NCBI ID   WP_173876702.1    Uniprot ID   A0A6M9F8K3
Organism   Streptococcus mitis strain S022-V3-A4     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2049445..2060743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M594_RS09840 (M594_09885) rplI 2050600..2051052 (-) 453 WP_000864214.1 50S ribosomal protein L9 -
  M594_RS09845 (M594_09890) - 2051049..2053022 (-) 1974 WP_173876700.1 DHH family phosphoesterase -
  M594_RS09850 (M594_09895) hpf 2053158..2053706 (-) 549 WP_004255322.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  M594_RS09855 (M594_09900) comFC/cflB 2053786..2054448 (-) 663 WP_173876701.1 ComF family protein Machinery gene
  M594_RS09860 (M594_09905) comFA/cflA 2054445..2055743 (-) 1299 WP_173876702.1 DEAD/DEAH box helicase Machinery gene
  M594_RS09865 (M594_09910) - 2055800..2056435 (+) 636 WP_173876703.1 YigZ family protein -
  M594_RS09870 (M594_09915) - 2056451..2056894 (+) 444 WP_173876704.1 PH domain-containing protein -
  M594_RS09875 (M594_09920) cysK 2056991..2057917 (+) 927 WP_173876705.1 cysteine synthase A -
  M594_RS09880 (M594_09925) tsf 2058039..2059079 (-) 1041 WP_173876706.1 translation elongation factor Ts -
  M594_RS09885 (M594_09930) rpsB 2059158..2059937 (-) 780 WP_007556349.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49611.39 Da        Isoelectric Point: 7.3257

>NTDB_id=418288 M594_RS09860 WP_173876702.1 2054445..2055743(-) (comFA/cflA) [Streptococcus mitis strain S022-V3-A4]
MKVNPNYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSAILEEWYLPIGAYYCRECLIMKRVRSDQSLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLIQAVDKQEPTLVHAVTGAGKTEMIYQVVAKVIDEGGAVCLASPRIDVCLELYKR
LQQDFSCEIALLHGESDPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYYAVKNSVKENGLRIFLTATST
DELDRKVRIGELKRLSLPRRFHGNPLIIPKPVWLSDFNHFLEKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQIKEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDK
PTGDLLFFHDGLNASIKKAIMEIQQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=418288 M594_RS09860 WP_173876702.1 2054445..2055743(-) (comFA/cflA) [Streptococcus mitis strain S022-V3-A4]
ATGAAAGTAAATCCAAATTATCTCGGTCGTTTGTTTACTGAGAATGAATTAACAGAAGAGGAACGTCAGTTGGCGGAGAA
ACTTCCAGCTATGAGAAAGGAGAAGGGGAAATTGTTCTGCCAACGTTGTAATAGTGCTATTCTAGAAGAATGGTATTTAC
CCATAGGTGCTTACTATTGTCGGGAGTGCTTAATTATGAAGCGAGTTAGGAGTGATCAATCTTTATACTATTTCCCACAG
GAGGATTTTCCGAAGCAAGATGTTCTTAAATGGCGTGGTCAATTAACCCCTTTTCAAGAGAAGGTGTCAGAGGGACTGAT
TCAAGCGGTAGACAAGCAAGAACCAACGTTAGTTCATGCGGTTACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCCAAAGTGATTGATGAAGGTGGTGCAGTATGTTTGGCCAGTCCTCGCATAGATGTTTGTTTGGAGCTATACAAGCGC
CTGCAACAGGATTTTTCTTGCGAGATAGCCCTCCTACATGGAGAATCAGATCCGTATTTTCGAACACCATTAGTTGTTGC
GACGACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ACAATCCAACGCTTTACTACGCTGTCAAGAATAGTGTAAAGGAGAATGGCTTGAGAATTTTTTTAACAGCGACTTCGACC
GATGAGTTAGATAGGAAGGTTCGCATAGGAGAACTAAAACGATTGAGCTTGCCAAGACGATTTCATGGAAATCCGTTGAT
TATTCCTAAACCTGTCTGGTTATCGGATTTTAATCACTTTTTAGAGAAAAATCGGTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTTATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGATAAAAGAAATT
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAAGATAGATTAGAGCAGGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATATTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGCCTGTTTACCAAGTCTAGCTTGATTCAGATTGGTGGACGAGTTGGGCGAAGCATGGATAAA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCAATTATGGAAATTCAGCAGATGAA
CAAGGAGGCTGGCTTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(293-396)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M9F8K3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

94.213

100

0.942
  comFA/cflA Streptococcus pneumoniae Rx1

93.981

100

0.94
  comFA/cflA Streptococcus pneumoniae D39

93.981

100

0.94
  comFA/cflA Streptococcus pneumoniae R6

93.981

100

0.94
  comFA/cflA Streptococcus pneumoniae TIGR4

93.75

100

0.938
  comFA/cflA Streptococcus mitis SK321

93.056

100

0.931
  comFA Lactococcus lactis subsp. cremoris KW2

50.117

99.306

0.498
  comFA Latilactobacillus sakei subsp. sakei 23K

38.106

100

0.382

Multiple sequence alignment