Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   E3S84_RS02370 Genome accession   NZ_CP047841
Coordinates   446081..446578 (+) Length   165 a.a.
NCBI ID   WP_029694050.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UP_644     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 441081..451578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3S84_RS02330 (E3S84_02330) - 441125..442111 (+) 987 WP_000161314.1 ROK family glucokinase -
  E3S84_RS02335 (E3S84_02335) - 442111..442440 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E3S84_RS02340 (E3S84_02340) - 442437..443060 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E3S84_RS02345 (E3S84_02345) comGA 443112..444086 (+) 975 WP_001633882.1 competence type IV pilus ATPase ComGA Machinery gene
  E3S84_RS02350 (E3S84_02350) comGB 444058..445128 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  E3S84_RS02355 (E3S84_02355) comGC 445142..445453 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E3S84_RS02360 (E3S84_02360) comGD 445431..445877 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  E3S84_RS02365 (E3S84_02365) comGE 445864..446163 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  E3S84_RS02370 (E3S84_02370) comGF 446081..446578 (+) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  E3S84_RS02375 (E3S84_02375) - 446675..446821 (+) 147 WP_001792109.1 hypothetical protein -
  E3S84_RS02380 (E3S84_02380) - 446811..447335 (+) 525 WP_001015120.1 shikimate kinase -
  E3S84_RS02385 (E3S84_02385) gcvT 447494..448585 (+) 1092 WP_000093348.1 glycine cleavage system aminomethyltransferase GcvT -
  E3S84_RS02390 (E3S84_02390) gcvPA 448605..449951 (+) 1347 WP_000019690.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  E3S84_RS02395 (E3S84_02395) gcvPB 449944..451416 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19199.92 Da        Isoelectric Point: 10.2192

>NTDB_id=417594 E3S84_RS02370 WP_029694050.1 446081..446578(+) (comGF) [Staphylococcus aureus strain UP_644]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIELRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTSFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=417594 E3S84_RS02370 WP_029694050.1 446081..446578(+) (comGF) [Staphylococcus aureus strain UP_644]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAACTTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CTTCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

96.97

100

0.97
  comGF Staphylococcus aureus N315

96.97

100

0.97

Multiple sequence alignment